In Vitro Evaluation of Antibacterial and Anticoagulant Activities of In Vitro Evaluation of Antibacterial and Anticoagulant Activities of Harmala Alkaloids Roots Harmala Alkaloids Roots
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Date
2023
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Journal of Bioresource Management
Abstract
Peganum harmala L. is a wild herbal plant rich in active compounds, and is among the most
used plants in local medicine. The purpose of this study is to contribute to the evaluation of
some biological activities of alkaloids extract of P. harmala roots. Using the classical method
of extraction of β-carboline alkaloids, the yield of alkaloids in the roots was estimated at 2.34
%. The thin layer chromatography (TLC) method identified the two components Harmine and
Hrmaline in the total alkaloid extract of the roots. We evaluated the antibacterial activity of
harmala alkaloids in roots against three referenced bacterial strains, and this using the agar
medium diffusion method, all extracts showed significant activity against all tested bacterial
strains, the largest inhibition zone diameter of 20 mm was recorded with Staphylococcus
aureus at a concentration of 50 mg/ml of crude alkaloids. The anticoagulant activity of
alkaloid extracts was also examined using the prothrombin time (PT) and partial activation
thromboplastin time (APTT) tests. The clotting times obtained in normal plasma indicate that
they have good activity in both coagulation pathways compared with sodium heparin,
especially in the prolongation of blood clotting in the intrinsic pathway.