Incidence de Listeria spp. et autres bactéries psychrotrophes dans le lait cru bovin dans le Nord Est Algérien
No Thumbnail Available
Date
2011-01
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
researchgate
Abstract
In this study a total of 104 milk samples collected from 60 dairy cattle in
six farms in the North East part of Algeria were examined for the presence
of Listeria spp. and other psychrotrophic bacteria. The isolation was accomplished
by cold storage of milk samples followed by plating on blood agar
added of Cefazoline. The isolates were identified phenotypicaly by studying
cultural, morphological, biochemical criteria; and genotypicaly using the
16S rRNA gene. Psychrotrophic bacteria from numerous genera have been
isolated, both gram negative “Acinetobacter spp., Aeromonas spp.,
Pseudomonas spp., Stenotrophomonas spp., Proteus spp., Yersinia spp.,
Klebsiella spp., Alcaligenes spp., and Flavobacterium spp.” and gram positive
“Enterococcus spp., Listeria spp., Staphylococcus spp., and Bacillus
spp.”. Enterococcus spp. was the dominant genera in different seasons. Milk
contamination by Listeria spp. was seasonal, its incidence was positive in
winter and in spring, however it was absent in summer and autumn.