Etude in vitro de l'effet antibactérien des associations d'antibiotiques
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Date
2012
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Abbes Laghrour University- Khenchela
Abstract
n order to evaluate the phenomena of bacterial resistance to antibiotics in aquatic ecosystems,
particularly in surface waters, it was interested to study Sebkhet Ouled M’barek of the City of
Khenchela, a water plan exposed to microbial pollution of fecal origin. In our study, we have
begun by estimating the microbiological quality of water using different research methods and
enumeration of test germs of fecal contamination. The results showed a significant microbial
pollution of our study area. Seven bacterial species were identified: Escherichia coli, Aeromonas
hydrophila, Methylobacterium mesophilicum, Shewanella putrefaciens, Comamonas testosteroni,
Staphylococcus epidermidis and Enterococcus faecium.
The study of antibiotic resistance showed, for E.coli, acquired resistance to Ampicillin and
Ticarcillin (by a low- level penicillinase) and to Chloramphenicol (by chloramphenicol
acetyltransferase). A. Hydrophila and the three other non- fermenting bacilli have a wild-type
resistance. S. epidermidis has acquired resistance to penicillin (by penicillinase plasmid),
Oxacillin and Erytromycin (by efflux mechanism). E. faecium have a wild-type resistance. The
study of the association of antibiotics on A. Hydrophila showed greater synergy between colistin
and cefotaxim. The technique of the chessboard allowed us to reveal, to these two antibiotics, the
lowest concentration giving a better effect on the growth of this species. They amount
respectively 166.5 and 6.25 µg / ml.