Enhanced Production and Quantitative Evaluation of Nigericin from the Algerian Soil-Living Streptomyces youssoufiensis SF10 Strain
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Date
2019-01-26
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MDPI
Abstract
Abstract: Nigericin, one of the main ionophoric polyethers produced by various Streptomyces
strains, presents relevant biological activities including antibacterial and recently studied antitumor
properties. This work describes the influence of different culture conditions on the production
of this metabolite by Streptomyces sp. SF10, isolated from a semi-arid soil sample collected at
Chélia Mountain, in Khenchela (Northeastern Algeria) and identified as Streptomyces youssoufiensis.
The extracts from the strain, cultured in a solid state or submerged fermentation conditions, using
several carbon sources at different pH values, in the presence or absence of iron (II) sulfate and
in co-culture with other Streptomyces species, were analyzed using a high-performance liquid
chromatography (HPLC) system equipped with an evaporative light scattering detector (ELSD).
The best culture conditions provided a concentration of nigericin of 0.490 0.001 mg/mL in
the extract. The HPLC-ELSD method, optimized here for the quantitative detection of nigericin,
can find wider applications in the analysis of several other metabolites characterized by a similar
polycyclic polyether structure or, more generally, by the lack of significant chromophores in their
molecular structure.