APPLICATION OF MALDI- TOF MS TECHNOLOGY TO THE IDENTIFICATION AND CHARACTERIZATION OF PATHOGENS IN DAIRY INDUSTRY
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Date
2018-03
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journalijar
Abstract
The prerequisite of any microbial identification technique, especially in
food industry, is its accuracy. Furthermore, it should be easy to
perform, fast and cost-effective to ensure safe products and identify
their microbial contamination. In this study, we tested Matrix-Assisted
Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry
(MALDI-TOF MS) as an efficient tool allowing identification of
bacterial isolates with high confidence and speed. Five bacterial species
were isolated from pasteurized milk samples collected from different
steps of their line production, in a local dairy company in Constantine,
Algeria. The isolates were analyzed by MALDI-TOF MS method and
results were compared with those provided by sequencing of the 16S
rDNA gene in order to confirm the identification. All bacterial isolates
were successfully identified by MALDI-TOF MS method to the species
level (scores ≥2). In parallel, 16S rDNA gene sequencing analysis
identified the five isolates to the species level (≥99% sequence
similarity). The results of MALDI-TOF MS-based identification of the
bacterial isolates studied showed a total concordance (100% same
species) for isolates designed S1, S2 and B2 to those of the reference
method (16S rDNA gene sequencing), except for two cases (isolate B1
and B3), were the identification by both proteomic and genomic
methods coincided at the genus level. This study suggests that
comparatively to 16S rDNA gene sequencing which provides limited
results; MALDI- TOF MS is a highly sensitive and rapid method that
could be used to identify pathogens in dairy industry.