A novel and ultrasensitive non‑enzymatic electrochemical glucose sensor in real human blood samples based on facile one‑step electrochemical synthesis of nickel hydroxides nanoparticles onto a three‑dimensional Inconel 625 foam

In this contribution, a simple and novel non-enzymatic electrochemical sensor for the detection of glucose was successfully prepared by direct in situ growth of nickel hydroxide nanoparticles (Ni(OH)2NPs) onto a three-dimensional Inconel 625foam (IN625F) substrate through a facile electrochemical route, using cyclic voltammetry (CV) method in alkaline medium without addition of nickel salts. Then, surface characterization of modifed Ni(OH)2/IN625F electrodes was carried out through advanced technologies, such as scanning electron microscopy (SEM) and X-ray difraction (XRD). The electrochemical catalytic behavior of the fabricated electrodes was investigated using CV and amperometric methods. The results revealed that the novel modifed sensor, Ni(OH)2/IN625F, showed the highest sensitivity of 5685 μAmM−1 cm−2 over a wide linear concentration range from 1 to10 mM, with lowest detection limit (LOD) of 2 μM (S/N = 3), and short response time within<2 s. Therefore, the proposed non-enzymatic electrochemical sensor demonstrated high selectivity and stability, good reproducibility, and low cost. In addition, analysis of human blood samples was performed. Hence, the constructed glucose sensor, Ni(OH)2/IN625F, with suitable performance could be used as a promising material in real human blood samples.