TOTAL PROTEIN POLYMORPHISM OF SOME ISOLATES OF FUSARIUM OXYSPORUM F. SP. ALBEDINIS USING SDS-PAGE
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Date
2021-06-19
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Pakistan Journal of Phytopathology
Abstract
Fusarium wilt caused by Fusarium oxysporum f.sp. albedinis (Foa), is one of the main diseases affecting date palm
(Phoenix dactylifera L.), and poses a constant threat to palm oases, especially in North African countries. In this study,
we performed electrophoresis of total proteins extracted from the mycelia of 20 Fusarium oxysporum f.sp. albedinis
and 03 isolates of Fusarium sp. Protein was extracted and separated by Sodium Dodecyl Sulphate Poly Acrylamide Gel
Electrophoresis followed by staining with silver nitrate. We noted variations in protein profile between the 23
Fusarium strains isolated from three regions Ghardaïa, Bechar and Adrar. These results demonstrate the presence of
important phenotypic polymorphism according to the ascending hierarchical classification of total proteins after the
analysis of the phonograms constructed by the JMP software (JMP SAS Pro (JMP®, Version <12>. SAS Institute Inc.,
2015).