Pathogenicity and biological control of Bayoud disease by Trichoderma longibrachiatum and Artemisia herba-alba essential oil
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Date
2018-04-29
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Journal of Applied Pharmaceutical Science
Abstract
All the Fusarium oxysporum f. sp. albedinis (Foa) isolated from rachis were pathogenic to the date palm seedlings
cultivar Deglet Nour, while Fusarium sp. isolated from soil (E1, E2, and E3) did not show any aggressiveness against
these seedlings. In vitro antagonistic effect of Trichoderma longibrachiatum against three isolates of Foa tested by
direct confrontation or remote confrontation on Potato Sucrose Agar (PSA) medium, revealed that the latest has
inhibited mycelial growth of the pathogen by more than 60%, compared to the control and this after an incubation
period of six days at 27 ± 2°C. Mycelial growth of T. longibrachiatum occupied the whole Petri dish after three days
of incubation while the three isolates of Foa (T15D, M15A1, and O15T) occupied only 17%, 11%, and 20%, of the
surface respectively. Biological control with essential oil of Artemisia herba-alba yielded important results with a
minimum inhibitory concentration (MIC) ranging from 2.5 to 5 μl/ml and a minimum fungicidal concentration (MFC)
of 80 μl/ml.