Elimination des nitrates par procédé biologique des eaux de la région de Khenchela

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Abbes Laghrour University- Khenchela
The purpose of this work is to study the phenomenon of biological denitrification by using mixed culture taken from activated sludge of sewage treatment plant of KHENCHELA and pure culture isolated from the same activated sludge. Our experiences were made in serial batch reactors incubated anaerobically in a candle jar at 30 ° C with several types of carbon sources. The first experiments with mixed cultures show different growth kinetics and denitrification. These kinetic depend directly to the nature, biodegradability and concentration of carbon source. The use of glucose allows bacteria to grow well with high denitrification yield. However, our experiments with pure cultures allows us to isolate six species identified as Pseudomonas luteola, Aeromonas hydrophilae, Enterobacter cloacae, Moraxella spp, Sphingomonas paucimobilis and Burkholderia cepacia,. They show the existence of bacterial species able to reduce nitrates with reduction rate rather important at different carbon sources.