Elimination des nitrates par procédé biologique des eaux de la région de Khenchela
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Date
2012
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Abbes Laghrour University- Khenchela
Abstract
The purpose of this work is to study the phenomenon of biological denitrification by
using mixed culture taken from activated sludge of sewage treatment plant of
KHENCHELA and pure culture isolated from the same activated sludge.
Our experiences were made in serial batch reactors incubated anaerobically in a candle jar at
30 ° C with several types of carbon sources.
The first experiments with mixed cultures show different growth kinetics and denitrification.
These kinetic depend directly to the nature, biodegradability and concentration of carbon
source. The use of glucose allows bacteria to grow well with high denitrification yield.
However, our experiments with pure cultures allows us to isolate six species identified as
Pseudomonas luteola, Aeromonas hydrophilae, Enterobacter cloacae, Moraxella spp,
Sphingomonas paucimobilis and Burkholderia cepacia,. They show the existence of bacterial
species able to reduce nitrates with reduction rate rather important at different carbon sources.