EFFECT OF PGPR INOCULATION ON DURUM WHEAT GROWTH VARIETIES
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Date
2020-09-15
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Global Science Publications
Abstract
Abstract – The purpose of this work is to isolate PGP-effect rhizobacteriaand to evaluate their potential for
durum wheat varieties. The isolation of the bacteria was done using the suspension-dilution process. Eleven
bacterial isolates were isolated from the rhizosphere of hard wheat. The latter were described by molecular
characterization, based on the sequencing of RNA 16s. Once established, these isolates were characterized
by their traits of growth promotion, such as biological control through the testing of the anti-fungal activity
which was performed to two species of Fusarium. The ability of stimulating growth of durum wheat varieties
was assessed in vivo. The molecular identification of eleven isolates showed 98-99 % of our isolates to
species of the following genera: Enterobacter, Enterobacter asburiae, Paenibacillus glucanolyticus and Serratia sp.
Research on the plant growth promoting traits in our isolates, showed that 80% of isolates have shown
positive results with qualitative and quantitative estimates of siderophores, as well as for solubilized
phosphate. All of our isolates showed a similar result with production of ammonium, cellulase, pectinase
and eight isolates released AIA at different concentrations; whereas only three isolates could release HCN,
and four isolates did not produce the protease. Similarly, direct inhibition of the growth of Fusariumsp
species was observed, with significant improvement in the different growth parameters of the durum wheat
varieties being studied. This research concluded that the findings obtained so far demonstrated that our
isolates have phytostimulative, and phytoprotective behaviors. The latter opens up the possibility of their
use in future laboratory studies in order to generate biofertilizer inoculants.