Total phenolic and flavonoids content, and in vitro antioxidant and antimicrobial activity of ethyl acetate and butanol extract from Senecio delphinifolius Vahl.
No Thumbnail Available
Date
2014
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Scholars Research Library
Abstract
The present work focuses essentially on the total phenolic and flavonoidscontents, and in vitro antioxidant and
antimicrobial activity of two fractions: ethyl acetate and n-butanol extracts of an Algerian endemic plant: Senecio
delphinifolius. The total phenolic content was determined using Folin-ciocalteu reagent to yield 7.22 ± 0.84 and
7.86 ± 0.25mg GA/mg respectively. The flavonoids contents were 22.96± 1.41 and 10.25 ± 8.94 mg Q/mg
respectively. Antioxidant activity was performed using DPPH reagent. Antioxidant activity ranged from 80.48 % to
73.91 %, using ascorbic acid as a control test. The antimicrobial activity of the two extracts was investigated using
a diffusion method against most common pathogenic bacterial strains: E. coli ATCC 2592, Pseudomonas
aerogenosa ATCC2783, Staphylococcus aureus ATCC 252923, Selmonella sp., Klebseilla pneumonia and one
fungus Candida albicans. The finding show that this extracts have a strong antioxidant activity and can be used as a
natural source of scavenging agents.