Ultrasound-Assisted Extraction, LC–MS/MS Analysis, Anticholinesterase, and Antioxidant Activities of Valuable Natural Metabolites from Astragalus armatus Willd.: In Silico Molecular Docking and In Vitro Enzymatic Studies
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Date
2022
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journal /Antioxidants
Abstract
The Astragalus armatus Willd. plant’s phenolic constituent extraction and identification
were optimized using the ultrasound-assisted extraction (UAE) method and the LC–MS/MS analysis,
respectively. Additionally, cupric reducing antioxidant capacity (CUPRAC), beta carotene, reducing
power, DMSO alcalin, silver nanoparticle (SNP)-based method, phenanthroline, and hydroxyl radical
tests were utilized to assess the extract’s antioxidant capacity, while the neuroprotective effect
was examined in vitro against acetylcholinesterase enzyme. This study accurately estimated the
chemical bonding between the identified phenolic molecules derived from LC–MS/MS and the
AChE. The extract was found to contain sixteen phenolic substances, and rosmarinic, protocatechuic,
and chlorogenic acids, as well as 4-hydroxybenzoic, hyperoside, and hesperidin, were the most
abundant substances in the extract. In all antioxidant experiments, the plant extract demonstrated
strong antioxidant activity and a significant inhibitory impact against AChE (40.25 1.41 g/mL).
According to molecular docking affinity to the enzyme AChE, the top-five molecules were found
to be luteolin, quercetin, naringenin, rosmarinic acid, and kaempferol. Furthermore, these tested
polyphenols satisfy the essential requirements for drug-like characteristics and Lipinski’s rule of five.
These results highlight the significance of the A. armatus plant in cosmetics, as food additives, and in
the pharmaceutical industry due to its rosmarinic and chlorogenic acid content.