Sélection des Bactéries Lactiques Productrices Des Bactériocines Isole a Partir d’un Produit Traditionnellement Fermente (Jben) de la Région de Khenchela.
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Date
2012
Journal Title
Journal ISSN
Volume Title
Publisher
Université Abbes Laghrour -Khenchela-
Abstract
Abstract
A total of 13 strains of lactic acid bacteria isolated starting from a traditionally fermented product (jben) was
identified on the basis of some number of morphological, biochemical and physiological criteria.
The identification as of these strains revealed the presence of too genera: Lactobacillus (77%), Lactococcus
(23%).
The screening of these 13 bacterial isolates for the production of antibacterial activities of the jben
allowed us to select 3 bacterial strains producers of bacteriocins they were the strains (Lactobacillus
plantarum ST2, Lactobacillus acidophilus TH1 and Lactococcus lactis Subsp. Lactis LCL2).
The research of bacterial antagonism was carried out according to the method of double-layers and
the method of wells diffusion agar under conditions that eliminate the effect of the lactic acid and hydrogen
peroxide. In addition, we highlighted the experimental conditions that eliminate the effect of the lactic and
hydrogen peroxide. In addition, we highlighted the experimental conditions to determine the spectrum of
activity.
The strains bacteriocin-producing and its bacteriocins show a potentiality in the fight against the
majority of the indicator bacteria tested (Gram+) in food systems particularly in the elaborate jben
traditionally under uncontrolled hygienic conditions.