Antibacterial Properties of Leaves and Peels Extracts of Citrus aurantifolia cultivated in Algeria against Multi-Drug Resistant Staphylococcus aureus Originating from Raw Milk
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Date
2023-11-01
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Tropical Journal of Natural Product Research
Abstract
Plants have an increased consumer preference and acceptability for the treatment of several
diseases. Here, the antibacterial properties of organic extracts obtained from leaves and peels of
Citrus aurantifolia from Algeria have been characterized. Two solvents, methanol, and ethanol,
were employed to extract the bioactive components. Quantitative analysis of total phenols and
flavonoids was conducted for the different extracts. The antibacterial activity was tested against
multidrug-resistant Staphylococcus aureus strains originating from raw milk and reference strains
including Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC
27853, Staphylococcus aureus ATCC 25923, and Bacillus cereus ATCC 11778. The results
revealed that leaves and peels extracts displayed significant antibacterial activity against the tested
bacteria. The inhibition zone diameters observed ranged from 16.00 mm to 22.00 mm. The highest
antimicrobial effect was observed with the ethanol extracts against the multidrug-resistant S.
aureus strains, as indicated by a minimum inhibitory concentration of 1.56 mg/ml. The highest
total phenolics and flavonoids contents were found to be 96 mg GAE/g and 54 mg QE/g in peels.
Hence, the reported results unveil valuable insights into the antibacterial effects of Citrus
aurantifolia extracts which have potential antimicrobial applications.