GSTM1 and GSTT1 polymorphisms, tobacco use as risk factors for nasopharyngeal carcinoma in maghreb population — A case-control study
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Date
2013-09-09
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Springer
Abstract
Abstract Nasopharyngeal carcinoma (NPC) has known environmental
risk factors, notably smoking, and enzymes that
biotransform carcinogens. The interindividual differences
observed in the metabolism of tobacco carcinogens substances
have been attributed to the genetic polymorphism of
genes, which code for enzymes involved in detoxification.
Polymorphic deletions of GSTM1 and GSTT1 genes
involved in the detoxification of potentially carcinogenic
agents may be a risk factor for NPC. To investigate the roles
of genetic variations of GSTM1 and GSTT1 in NPC susceptibility
in the Northern African population, we conducted a
case–control study of 132 NPC cases and 200 controls.
DNA was isolated from blood for cases and controls.
GSTM1 and GSTT1 deletion variants were genotyped by
multiplex PCR assays. Allelic frequencies between the two
groups were compared using a χ2 test, and odds ratio (OR)
with 95% confidence intervals were calculated. The results
indicate that an excess of the GSTM1 null genotype was
observed in cases compared with controls, 50% versus 33%.
The genotypic frequency of GSTM1 null genotype between
controls and patients was significantly different. This genotype
confers an increased risk of NPC [OR = 2.18; CI 95%
(1.23–5.33). The association between GSTM1 and increased
NPC risk in this cases-controls study was present among both
men [OR = 2.59 ; CI95% (1.81–4.32)] and smokers. No significant
association was observed between null GSTT1 genotype
and the risk of CNP.