The Investigation of the Oxidative Stress-Related Parameters in Type 2 Diabetes Mellitus
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Date
2024-03-06
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elsevier
Abstract
Objective: Oxidative stress, defined as an imbalance between reactive oxygen species production and
breakdown by endogenous antioxidants, is closely associated with diabetes mellitus. The diabetes is
characterized by hyperglycemia together with biochemical alterations of glucose and lipid peroxidation.
Oxidative stress has been implicated in the pathogenesis of type 2 diabetes and its complications.
Methods: This study was conducted to investigate the variation in oxidative stress-related parameters in
type 2 diabetes. Blood serum samples were collected from diabetes patients and nondiabetes healthy
controls. Glucose concentrations, levels of glycated hemoglobin (A1C) and serum oxidative stress
markers (glucose-6-phosphate dehydrogenase [G6PDH], malondialdehyde [MDA], glutathione [GSH],
glutathione reductase [GR], glutathione peroxidase [GPx] and superoxide dismutase [SOD]) were
estimated.
Results: Fasting serum glucose concentration in type 2 diabetes patients of both sexes was increased
significantly as compared with the healthy controls. Level of A1C was greater than standards. Significant
elevation in MDA level and depletion in GSH content were observed in diabetes patients in comparison
with controls. The diminution in G6PDH activity was accompanied in part by a decrease in the antioxidative
enzymes activities (GPx and GR), and in part by an increase in SOD activity in all diabetes
patients as compared with the control group. The regression analysis showed no correlation between
diabetes duration and severity of oxidative stress; however, there was a significant association between
A1C and severity of oxidative stress.
Conclusions: The present study shows that there is an oxidative stress state in type 2 diabetes patients
compared with healthy subjects. Our data suggest that chronic hyperglycemia causes a significant change