Phytochemical Screening and Effect of Three Plants Collected from the Region of Khenchela, Algeria against Multi-drug Resistant Pathogenic Bacteria
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Date
2022-03-27
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Indian Journal of Novel Drug Delivery
Abstract
This work aims to perform phytochemical analysis of hydroethanolic extracts of
three medicinal plants commonly used in folk medicine (Juniperus oxycedrus,
Eucalyptus globulus, and Matricaria chamomilla) and to evaluate their
antibacterial potential against multi-drug resistant bacteria. Disc diffusion method
has been used to determine the antibacterial activities and minimum inhibitory
concentrations (MIC) of different plant extracts against four Gram negative
bacteria (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and
Enterobacter aerogenes) and two Gram positive bacteria (Staphylococcus aureus
and Bacillus subtilis). Antibiotic sensitivity test was performed in Mueller Hinton
agar with three commonly used antibiotics (tetracycline, vancomycin and
oxacillin). Phytochemical analysis revealed the presence of tannins and free
quinones in all the extracts, whereas alkaloids were absent. The results of
antibiogram revealed that all strains were resistant to tested antibiotics with
exception of Staphylococcus aureus that was found to be susceptible to oxacillin.
Also, obtained results showed that hydroethanolic extracts exhibited dosedependent
growth inhibitory effects against Staphylococcus aureus and Bacillus
subtilis, while no inhibitory effect on Gram negative bacteria was observed. In
conclusion, the present work provides useful baseline information for the
potential use of the studied plants in the fight against MDR strains.