Antioxidant and antibacterial activity of extract and phases from stems of Spartium junceum L. growing in Algeria
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Date
2016
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International Journal of Phytomedicineb
Abstract
A b s t r a c t
This work aimed to evaluate the antioxidant and antibacterial activities of the hydroalcoholic
(80% methanol) extract and n-hexane (n-Hex), chloroform (Chl), ethyl acetate (EtAc), and nbutanol
(n-But) phases from Spartium junceum L. stems collected in Algeria. Preliminary
phytochemical investigations on phenolic compounds have been carried out.
The total phenolic content, spectrophotometrically determined, ranged from 71.8095 ± 3.7136
mg GAE/g (extract) to 0.0582 ± 0.0106 mg GAE/g (n-Hex). By HPLC-PDA analysis flavonoids
(flavone derivatives), p-hydroxybenzoic acid, p-hydroxycinnamic acid, and cinnamic acid
derivatives were identified both in the extract and phases.
S. junceum extract showed a noticeable free radical scavenging effect in the DPPH test (IC50 =
0.6833 ± 0.0240 mg/mL), mild reducing power, and strong chelating activity (IC50 = 0.2292 ±
0.0138 mg/mL). Among the phases, n-But displayed the best effect both in the DPPH test and
reducing power assay, whereas n-Hex resulted the most active in the ferrous ions chelating
activity assay. A positive relationship between DPPH radical scavenging activity and total
phenolic content was found. Both the extract and phases exhibited antimicrobial activity against
Gram-positive bacteria only. Staphylococcus aureus ATCC 6538 was the most susceptible
strain (MIC range: 15.60-250.00 μg/mL), and the Chl phase showed the greatest efficacy. S.
junceum extract resulted non-toxic against Artemia salina.
The obtained results demonstrate the potential of S. junceum stems as safe sources of natural
antioxidant and antimicrobial compounds