Evaluation of putative cytotoxic activity of crude extracts from Onopordum acanthium leaves and Spartium junceum flowers against the U-373 glioblastoma cell line
No Thumbnail Available
Date
2015-07
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
researchgate
Abstract
Abstract: Crude hydromethanolic (80% methanol) extracts produced by maceration of Onopordum acanthium leaves
and Spartium junceum flowers were tested for cytotoxic effects against glioblastoma U-373 tumour cells. Onopordum
acanthium extract was found to be ~5 times more cytotoxic than Spartium junceum (IC50 values of 309 and 1602μg/ml,
respectively). Similar to most chemotherapeutic agents killing through the intrinsic pathway, Onopordum killed the cells
via apoptosis, which was confirmed by the activation of caspase-3. Spartium exerted its weak cytotoxic effect,
presumably by a caspase-independent, non-apoptotic form of necrotic-like programmed cell death. Onopordum
acanthium is considered a promising plant for the researchers investigating putative biological activities, particularly
antitumour and immune-related activity