In Vitro Antimicrobial Activity of Mentha piperita Leaves Extracts
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Date
2022-12-26
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Indian Journal of Novel Drug Delivery
Abstract
M. piperita (Lamiaceae family) have been used as traditional remedies for the
treatment of several diseases. In this work, we aimed to characterize the
antimicrobial activity of extracts of Mentha piperita leaves. Various extracts
(methanolic and aqueous) were analysed for their phenolic content and
antimicrobial activity. This was done by the method of diffusion against three
Gram+ (Staphylococcus aureus ATCC 25923, Methicillin-resistant Staphylococcus
aureus (MRSA) ATCC 43300 and Bacillus subtilis ATCC6633) and three Gram-
(Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922 and
Salmonella typhi ATCC 14028) strains and a yeast (C. albicans ATCC 1024). On the
other hand, the minimum inhibitory concentration (MIC) and the minimum
bactericidal concentration (MBC) of active extracts; were determined by using the
dilution method on solid medium. The results indicate that the leaves extracts are
rich in polyphénols. On the other side, the Antimicrobial tests exhibited different
activities depending on the strain used and the nature of the extract (methanol or
aqueous). MRSA was the most sensitive since its growth was inhibited by both
extracts with the widest diameter of 23.5mm, whereas Gram-negative bacteria and
the yeast C. albicans were the most resistant. The results of the MIC and the MBC of
active extracts showed that both extracts (methanolic and aqueous) of leaves
showed the best activity against SARM (MIC: 8mg/ml; MBC: 10mg/ml).
Determination of total phenols and flavonoids suggested that the antibacterial
activity is attributed mainly to the richness of this plant in phenolic compounds.
These preliminary results may justify the use of this plant in the treatment of
certain bacterial diseases and that phenolic compounds may be exploited for
therapeutic purposes, especially antibacterial.