Optimization of extraction process and characterization of water-soluble polysaccharide (Galactomannan) from Algerian biomass; Citrullus colocynthis seeds
No Thumbnail Available
Date
2018
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
In this work, the optimization of extraction of polysaccharides from Citrullus
colocynthis curd polysaccharides (CCPs) is investigated by using the analysis
of variance. For this aim, a chemical extraction method has been developed
in order to be able to isolate CCPs at high level of purity. The extraction was
performed by using boiling water at different extraction times (30, 60 and
120 min), extraction temperatures (50, 75 and 100°C), and a material ratio
(30, 60 and 100 g/ml), for maximum polysaccharide extraction. The 3-D
response surface plot and the contour plot derived from the mathematical
models are used to determine the optimal conditions. The optimum
extraction conditions are as follows: extraction temperature 100°C, extrac-
tion time 120 min and the ratio of water 30 ml/g. Under these conditions, the
experimental final mass is 210.6 mg which is very close to the predicted
value (209.46 mg) with a regression coefficient of 94.01%. In addition, the
existence of the polysaccharide has been confirmed by the characterization
of the galactomannan obtained from C. colocynthisis obtained by Fourier
transform infrared-attenuated total reflection, IR-Raman spectroscopy and
X-ray diffraction.