Occurrence and spread of beta-lactamases-producing Enterobacteriaceae isolated from river receiving treated effluent of wastewater treatment plant
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Date
2019-01-07
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Desalination and Water Treatment
Abstract
Rivers receiving effluents from urban wastewater treatment plants are suspected to be among
the main sources for the development and dissemination of multidrug-resistant bacteria into the
environment. In the present study, we analyzed 15 river samples in order to assess the spread of
extended-spectrum
β-lactamase (ESBL)-producing Enterobacteriaceae. One hundred eighty-eight
Enterobacteriaceae were identified and classified as members of the genera Pantoea, Klebsiella, Escherichia,
Enterobacter, Serratia, Yersinia, Providencia, and Shigella. Based on susceptibility results, the most part
of isolates were highly resistant to the tested β-lactams (AMX, TIC AMC, and ATM), first-generation
cephalosporins (CL), and second generation (FOX). ESBL production was determined by different
methods, concluding its presence in 31.38% of the isolates by the disc approximation method, 25%
by double-disk synergy test, and 28.72% by double-disk test. Given this situation, there is an urgent
need to make more attention to the contamination of urban river by ESBL-producing bacteria, which
constitute the main source of community infection