CONTRIBUTION DU DIAGNOSTIC BIOCHIMIQUE BACTERIEN DANS L’ETABLISSEMENT DES PARENTES PHYLOGENETIQUES : CAS DES Enterobacteriaceae ET Pseudomonas sp
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Date
2011-12
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Sciences& Technologie
Abstract
Ten bacterial strains were isolated from clinical samples provided by the bacteriology laboratory (Daksi, Constantine).
Among the ten strains studied, eight belong to the Enterobacteriaceae and two are among the Pseudomonadaceae. These
strains were presumptively identified by their biochemical characteristics, susceptibility or resistance to antibiotics, and
total nitrogen measured by the Kjeldahl method. A phylogenetic profile was produced by phenetic methods based on the
calculation of genetic distances using the Neighbor Joining algorithm (NJ) and bioinformatics analysis of 16S rRNA
sequences to compare the two phylogenies (phenotypic and genotypic). The analysis of topologies obtained showed that the
biochemical phenotype has some limitations in bacterial identification and sequence analysis of 16S rRNA is a key tool in
bacterial identification across the case.