Protective role of L-cysteine against nickel induced hepatotoxicity in Albino Wistar rats
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Date
2018-11-30
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J Pharmacol Med Chem
Abstract
OBJECTIVE: The present study was undertaken to investigate the
hepatoprotective effect of L-cysteine on nickel-induced oxidative stress in
experimental rats.
METHODS: Male albino (Wistar) rats were divided into four groups of seven
each: the first group was used as controls. The second group was given orally
L-cysteine at dose of 100 mg/kg b.wt. The third group was administrated
intraperitoneally with nickel sulfate at dose of 20 mg/kg and the fourth one
given both L-cysteine and nickel for three consecutive weeks. Liver function
markers alanine aminotransferase (ALT), aspartate aminotransferase
(AST), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH), total
bilirubin and proteins in serum and hepatic malondialdehyde (MDA) and
antioxidants parameters including glutathione (GSH), superoxide dismutase
(SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) were measured.
RESULTS: Nickel treatment produced oxidative liver injury characterized
by an increase in hepatic markers enzymes (ALT, AST, ALP, LDH) activities
and bilirubin level with a reduction in total proteins concentration.
Simultaneously, it led to an increase of MDA level with a reduction of
GSH concentration, SOD, CAT and GSH-Px activities. These results are
also substantiated with obviously changes in hepatohistology. However, the
treatment with L-cysteine significantly ameliorated the previous parameters
and resulted in an improvement of the histopathological hepatic lesions.
CONCLUSION: Depending on the findings, it can be concluded that
L-cysteine possesses a potential antioxidant power effect against nickel
hepatotoxicity.