Browsing by Author "Yasmine Arab"

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    Antimicrobial, antibiofilm, anti-quorum sensing and motility inhibition activities of essential oil from seeds of food spice Xylopia aethiopica (Dunal) A. Rich. on some pathogenic bacteria
    (Research Journal of Biotechnology, 2021-06) Yasmine Arab
    Abstract Medicinal food excipients can be used to combat microbial infections especially essential oils. Xylopia aethiopica (Dunal) A. Rich is a highly consumed medicinal food spice in Africa and its seeds essential oil was extracted by hydro-distillation and characterized by GC-FID and GC-MS. Its antimicrobial, anti-biofilm, anti-motility and quorum sensing inhibition potentials were evaluated on some pathogenic microorganisms. The identified compounds were grouped as oxygenated monoterpenes (57.06%) and monoterpenes hydrocarbons (28.96%) as major components while sesquiterpene hydrocarbons (5.94%), oxygenated sesquiterpenes (2.29%) and diterpenes (1.79%) where minor constituents. The most abundant constituent in the essential oil is myrtenol (13.25%), an oxygenated monoterpene. The most sensitive gram-positive and gram-negative bacteria were S. aureus and P. aeruginosa respectively with MIC values of 0.3125 μg/mL while the yeast C. albicans showed MIC of 0.625 μg/mL. Good antibiofilm results were found with highest inhibition percentage in S. aureus varying from 73.0±3.0 at MIC to 9.0±0.5 % at MIC/32. Biofilm inhibitions were higher in gram-positive bacteria than for gramnegative and yeast. Highest motility inhibitions were 45.32±0.10 and 63.84±3.50% in swimming and swarming models respectively at dose of 100 μg/mL. The essential oil of X. aethiopica showed good antiquorum sensing activity with quorum-sensing inhibition zones of 22.0±0.5 mm at MIC. The good results show that consumption of X. aethiopica is potent biocontrol means to reduce severity and virulence of food pathogens and to reduce their resistance to antibiotics which is a global health problem
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    Assessment of in vitro activities and chemical profiling of Senecio hoggariensis growing in Algerian Sahara
    (BIODIVE RS ITAS, 2022-07) Yasmine Arab
    Abstract. Arab Y, Sahin B, Ceylan O, Zellagui A, Olmez OT, Kucukaydin S, Tamfu AN, Ozturk M, Gherraf N. 2022. Assessment of in vitro activities and chemical profiling of Senecio hoggariensis growing in Algerian Sahara. Biodiversitas 23: 3498-3506. The in vitro antioxidant, anticholinesterase, tyrosinase inhibitory, antibiofilm, and anti-quorum sensing activities of the ethyl acetate extract of Senecio hoggariensis, growing in Algerian Sahara, were studied along with its chemical constituents using HPLC-DAD. The chromatographic analysis unveiled seven phenolic compounds, including p-coumaric acid as a major component. Additionally, the extract showed moderate DPPH radical scavenging activity, compared to known standards. At 200 μg/mL, the extract disclosed equitable acetylcholinesterase (AChE), butyryl-cholinesterase (BChE) and tyrosinase inhibition rates with respective values of 37.01±1.48%, 18.87±4.18% and 45.99±1.81%. Likewise, the extract exhibited a good antibiofilm activity against Candida albicans ATCC 10239 biofilm production with an inhibition ratio of 59.56±0.40%, at 50μg/mL. The anti-quorum sensing by QS-regulated violacein pigment production inhibition test was determined using Chromobacterium violaceum CV026 and CV12472. The swarming motility inhibition assay was determined using Pseudomonas aeruginosa PA01. It is evident from the findings that Senecio hoggariensis could be considered potential antioxidant, anti-QS, and antibiofilm compounds. However, the origin of anti-biofilm and anti-quorum sensing activities of the ethyl acetate extract could be revealed by further studies on the mechanism of action of active compounds that can be isolated via activity-guided fractionation.
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    GC/MS Analysis and Antioxidant Activity of the Essential Oil leaves Thymus numidicus Poiret. growing in Mila
    (Journal of New Technology and Materials, 2014-06-26) Yasmine Arab
    Abstract Essential oil components of the leaves of Thymus numidicus growing in Mila have been studied by gas chromatography mass spectrometry GC/MS to afford 60 Sixty compounds representing 94.0 % of the total oil and mainly represented by oxygenated monoterpenes,. The main constituents of the essential oil from the leaves were thymol (41.2%), β-cymene (12.9%), chlorocresol (11.2%), β-linalool (10.7%) and methyl thymol ether (3.3%). The antioxidant activity was evaluated by spectroscopic method, used for that free radical compound (DPPH) and in comparison with vitamin C as a standard. The essential oil showed a moderate activity against free radical compound (DPPH) 17.4% at 1M.
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    HPLC-DAD Phenolic Composition, Antioxidant, Anticholinesterase, Antidiabetic and Anti-quorum Sensing Properties of Bitter Kola (Garcinia kola) and Kolanut (Cola acuminata)
    (Pharmacognosy Research, 2023-02-06) Yasmine Arab
    ABSTRACT Background: Eating Cola acuminata and Garcinia cola nuts in African societies symbolizes in socio-cultural hospitality. They stimulate the nervous system, reduce fatigue and sleep. Objectives: To determine the phenolic composition and bioactivities of G. kola and C. acuminata. Materials and Methods: Hydro-ethanol extracts of the nuts were prepared and their phenolic profiles determined using HPLC-DAD. Antioxidant, anticholinesterase, antidiabetic, antimicrobial, antibiofilm and anti-quorum sensing properties were determined. Results: The most abundant phenolic compound was caffeic acid (105.4±0.75 mg/g) in C. acuminata and myricetin (277.2±0.90 mg/g) in G. kola. The extracts showed good antioxidant activity in five complementary assays and G. kola was more active than both α-tocopherol and BHA standards in the DPPH•, CUPRAC and ABTS•+ assays while C. acuminata was more active than only the α-tocopherol standard in the same assays. Activities were close to those of standards in the β-Carotene-linoleic acid and metal chelation assays. Both extracts had good inhibition of Butyrylcholinesterase (BChE) and Acetylcholinesterase (AChE) with IC50 values 63.27±0.98 μg/mL and 94.15±1.05 μg/mL for C. acuminata and G. kola respectively compared to 5.50±0.25 μg/mL for galantamine in the AChE assay. In the BChE assay, the inhibitory activity was higher for G. kola (IC50 = 38.66±0.80 μg/ mL) that the standard galantamine (IC50 = 42.20±0.48 μg/mL) while that for C. acuminata (IC50 = 87.31±0.77 μg/mL) was moderate. The extracts inhibited α-amylase and α-glucosidase with G. kola (IC50=18.43±0.74 μg/mL) being more active than standard acarbose (IC50=20.52±0.84 μg/mL in the α-glucosidase assay. The nuts could inhibit expression of virulence factors in Chomobacterium violaceum CV12472 by disrupting violacein production and flagellated Pseudomonas aeruginosa PA01 by disrupting swarming motility. Conclusion: The results indicate good nutraceutical potential of both nuts.
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    Total phenolic and flavonoids content, and in vitro antioxidant and antimicrobial activity of ethyl acetate and butanol extract from Senecio delphinifolius Vahl.
    (Scholars Research Library, 2014) Yasmine Arab
    The present work focuses essentially on the total phenolic and flavonoidscontents, and in vitro antioxidant and antimicrobial activity of two fractions: ethyl acetate and n-butanol extracts of an Algerian endemic plant: Senecio delphinifolius. The total phenolic content was determined using Folin-ciocalteu reagent to yield 7.22 ± 0.84 and 7.86 ± 0.25mg GA/mg respectively. The flavonoids contents were 22.96± 1.41 and 10.25 ± 8.94 mg Q/mg respectively. Antioxidant activity was performed using DPPH reagent. Antioxidant activity ranged from 80.48 % to 73.91 %, using ascorbic acid as a control test. The antimicrobial activity of the two extracts was investigated using a diffusion method against most common pathogenic bacterial strains: E. coli ATCC 2592, Pseudomonas aerogenosa ATCC2783, Staphylococcus aureus ATCC 252923, Selmonella sp., Klebseilla pneumonia and one fungus Candida albicans. The finding show that this extracts have a strong antioxidant activity and can be used as a natural source of scavenging agents.