Browsing by Author "Supervisor:Fercha Azzedine"

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    USE OF SEAWEED AND PLANT EXTRACTS TO IMPROVE CALLUS INDUCTION IN TOMATO
    (Abbes LaghrourUniversity,Khenchela, 2024) Belmekki Amira; Allaoui Rima; Supervisor:Fercha Azzedine
    This research aimed to investigate the impact of extracts from three medicinal plants (Syzygium aromaticum, Rhaponticum acaule, Rhamnus lycioides) and seaweed extract (Dalgin) on the germination and callus formation in tomato plants (Super strain B). The findings demonstrated that pre-treating seeds with these plant extracts positively influenced seed germination and the growth of tomato seedlings in vitro. Additionally, callus culture studies revealed that growing callus with a combination of auxins and cytokinins (0.2 ppm NAA & 2 ppm BAP) yielded better results compared to using leaves. The extracts, including commercial seaweed extract, had varying but generally positive effects, especially in the absence of hormones. However, the Tafgha plant extract exhibited the most significant effect on the speed and mass of callus formation when hormones were present. These beneficial effects are likely due to the chemical composition of the extracts, such as those found in the seaweed extract. Further studies and detailed chemical analyses are needed to confirm these effects and to explore the mechanisms by which plant extracts enhance growth and callus induction in tomatoes and other crops.
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    USE OF PLANT EXTRACTS TO MITIGATE SALT STRESS IN TOMATO (SOLANUM LYCOPERSICUM L.)
    (Abbes Laghrour University,Khenchela, 2024) Zemaili Aya; Lachekhab Meriem; Supervisor:Fercha Azzedine
    This research aims to investigate the impact of pre-treatment or irrigation using extracts from three medicinal plants (Syzygium aromaticum, Rhaponticum acaule, and Rhamnus lycioides) on alleviating the adverse effects of salt stress on the germination and growth of the Heinz 1530 tomato variety. The findings indicated that moderate saline stress (100 mM NaCl during germination and 50 mM NaCl during the seedling stage) significantly and negatively affected most studied parameters. However, the application of plant extracts either as a pre-treatment or through irrigation mitigated the negative impacts of salinity, enhancing the salinity tolerance of tomato seedlings. While the results are promising, further studies are necessary to elucidate the mechanisms by which these biostimulators influence salinity tolerance and plant growth.