Browsing by Author "Samira Bouhalit"

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    Ameliorating Role of Ulva Lactuca Extract on Neurobehavioral and Neurochemical Abnormalities Caused by Lithium in Rats
    (Journal of Bioresource Management, 2021-12-31) Samira Bouhalit
    Lithium is a relevant mood-stabilizing agent that is used in the management of depressive and manic episodes in bipolar, disorder; however, its application may cause diverse side effects including oxidative stress. This work investigated the neuroprotective and anti-stress activity of Ulva lactuca extract (MEUL) against lithium induced neuronal toxicity in rats. Lithium (2 g/kg) was administered in diets with or without MEUL (250 mg/kg BW by gavage) for 90 days. Lithium induced oxidative stress lead to an elevation in lipid peroxidation and oxidation protein product levels, a reduced superoxide dismutase, glutathione peroxidase activities, glutathione, and ascorbic acid concentrations in brain. The level of brush border enzymes (Ca2+ATPase, Mg2+ATPase) and acetylcholinesterase activity was reduced after lithium treatment. Histopathological observations confirmed these results. Open field and elevated plus maze behavioral tests showed an impaired recognition memory. Treatment with MEUL alleviated neurobehavioral alterations, attenuated oxidative stress markers and restored these biological parameters to normal standards, as well as a histological improvement. Ulva lactuca extract offers neuroprotection, against lithium-induced behavioral and oxidative brain damages.
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    ASSESSMENT OF THE POTENTIAL ROLE OF L- METHIONINE ON NICKEL SULFATE INDUCED RENAL INJURY AND OXIDATIVE STRESS IN RAT
    (Innovare Academic Sciences, 2018-04-16) Samira Bouhalit
    ABSTRACT Objective: The study was designed to investigate the possible protective role of methionine (Met) in nickel sulfate induced oxidative stress in renal tissue. Methods: Rats were divided into the following groups: Healthy control; L-methionine (Met, 100 mg/kg, orally); nickel sulfate (Ni, 20 mg/kg, intraperitoneally); and combination Ni+Met. The experiment lasted 21 days. Antioxidant and renal function parameters with histological study were performed. Results: In the Ni group, marked renal damage was noticed with the significant elevation in the levels of creatinine, urea, and uric acid in serum. Animals also showed a significant rise in the lipid peroxidation level with a concomitant decrease in glutathione (GSH) and various antioxidant enzymes GSH peroxidase, catalase, and superoxide dismutase. Moreover, these changes in rats given the combined therapy nickel plus methionine were significantly less than those of group nickel, met prevented these alterations and maintained the antioxidant status. Histopathological examination of the kidney tissue proved the protective effect of Met against nickel toxicity. Conclusions: These results demonstrated that methionine augments antioxidant defense against nickel-induced toxicity and provides evidence that it has therapeutic potential as a renoprotective agent.
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    Effect of Ruta chalepensis on Zinc, Lipid Profile and Antioxidant Levels in the Blood and Tissue of Streptozotocin-Induced Diabetes in Rats Fed Zinc-Deficient Diets
    (elsevier, 2017-08-15) Samira Bouhalit
    In diabetes, oxidative stress and lipid abnormalities are common and pronounced and represent important factors that are involved in the development of complications of diabetes. Zinc deficiency generally induces oxidative stress, but it is well known that the antioxidant Ruta chalepensis has an effective modulator role in oxidative stress in metabolic diseases. The aim of this study was to investigate the effect of R. chalepensis extract on blood biochemical parameters, tissue zinc status and antioxidant systems in rats with diabetes that were fed zinc-deficient diets. We divided 28 male albino Wistar rats into 4 groups: 2 groups, 1 group with diabetes, 1 group without diabetes, were fed zinc-sufficient diets, while the other 2 groups of rats with diabetes were fed zincdeficient diets. One group was not treated, and the other was treated with the extract of R. chalepensis. After 3 weeks of dietary manipulation, the fasting animals were killed. The body-weight gains of the zinc-deficient animals with diabetes were lower than those of the zinc-adequate animals with diabetes. It was noticed also that inadequate dietary zinc intake increased the glucose, cholesterol, triglyceride, urea, uric acid, creatinine and lipid peroxidation levels. In addition, the zinc-deficient diet led to a decrease in zinc tissues (femur, liver, kidney), glutathione concentration and both glutathione peroxidase and glutathione-S-transferase activities. However, R. chalepensis treatment ameliorated all the previous parameters approximately to their normal levels. It seems that R. chalepensis supplementation is a potent factor in reducing the oxidative severity of zinc deficiency in experimental diabetes through its hypoglycemic and antioxidant actions. © 2017 Canadian Diabetes
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    EFFECT OF SILYMARIN EXTRACTED FROM SILYBUM MARIANUM ON NICKEL HEMATOTOXICITY AND NEPHROTOXICITY IN MALE ALBINO WISTAR RATS
    (International Journal of Pharmacy and Pharmaceutical Sciences, 2017-06-19) Samira Bouhalit
    Objective: The objective of this study was to investigate the effect of silymarin extract from Silybum marianum against nickel-induced alterations in haematological indices, kidney dysfunction and renal antioxidant defence system. Methods: Male albino Wistar rats were divided into four groups seven each. Control, silymarin, nickel and nickel plus silymarin. Silymarin was administrated orally (100 mg/kg b. wt) and nickel as nickel sulfate (NiSO4 6H2 Results: The treatment with nickel led to a significant decrease in body weight with an increase in both absolute and relative kidney weights and a significant increase in renal markers, which confirmed by histopathological alteration. A microcytic anemia was also observed, which was manifested by a reduction of red blood cells count (RBC), hemoglobin (Hb) concentration, platelet counts (Plt), hematocrit and white blood cells counts (WBC). The level of lipid peroxidation was increased. Whereas, GSH concentration and enzymatic antioxidants SOD, GSH-Px and CAT activities were decreased. The co-treatment with methanolic extract of milk thistle attenuated the variation in the hematological and renal markers, decreasing renal lipid peroxidation (p<0.05) with a concomitant increasing reduced glutathione content (p<0.01) and restoring the antioxidant enzymes (SOD, CAT, GSH-Px) in kidney, as well as an improvement in histological changes compared to those previously noticed in nickel group. 0) was given intraperitoneally (20 mg/kg b. wt) at alternative days. The experiment continued for three consecutive weeks. Body weight was recorded regularly. After overnight fasting, animals were killed and serum creatinine, serum urea, serum uric acid, hematological parameters and renal antioxidant markers were determined. Conclusion: To conclude, these findings demonstrated that silymarin extract effectively improved heamatotoxicity and nephrotoxicity caused by nickel.
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    In vitro assessment of the bioactive potential of Artemisia campestris L. fractions growing in Khenchela (Algeria)
    (South Asian J, 2022-05-17) Samira Bouhalit; Derouiche Faouzia; Douaouya Liliaa; Djemil Randab; Kara Ali Wahiba
    In order to valorize Algerian medicinal plants, this work aims to characterize quantitatively and qualitatively Artemisia campestris L. fractions, a medicinal plant from the Aures-Algeria area, and investigate its biological activities in vitro. During preliminary phytochemical screening, flavonoids, saponins, tannins, alkaloids, reducing compounds, and coumarins were discovered. Total polyphenols and flavonoids are greater in the n-butanolic fraction than in ethyl acetate and petroleum ether. The resulting antioxidant capability was assessed in vitro using three methods: DPPH free radical scavenging, hydrogen peroxide trapping, and iron reduction. The n-butanolic extract suppresses DPPH oxidation with an IC50 of 2.239±0.32mg/ml, which is comparable to that of standard (1.824±0.97mg/ml). Despite the fact that similar findings were seen in the neutralization of hydrogen peroxide and the iron chelating activity, The anti-inflammatory action was proven in vitro by inhibiting protein denaturation and increasing HRBC membrane stability (Human Red Blood Cells). The n-butanolic fraction was more effective than diclofenac in preventing BSA degradation. It also inhibited membrane hemolysis in human erythrocytes by up to 83%. Activated partial thromboplastin and prothrombin times were used to analyze extrinsic and intrinsic coagulation pathways in A. campestris in order to determine its anticoagulant activity. The n-butanol fraction had the greatest impact on PT and aPTT lengthening, with 75.2s and 351s at 3 mg/ml concentration, respectively. The n-butanolic fraction of the A. campestris aerial part exhibited antioxidant, anti-inflammatory, and anticoagulant activities. As a result, it may be a viable natural resource for mitigating the impact of stress, which causes inflammatory and cardiovascular disorders.
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    Protective Effect of Silymarin Extracted from Silybum marianum Seeds upon Nickel-Induced Hepatotoxicity in Albino Wistar Rats
    (Annals of Microbiology and Immunology, 2018-09-11) Samira Bouhalit
    Nickel cytotoxicity results in high oxygen species production, which leads to continuous injury and dysfunction of different organs including liver. Thus, the present study was conducted to investigate the protective effects of silymarin on nickel-induced hepatotoxicity. Male rats were divided into four groups of seven each: Control, silymarin (Sil), nickel (Ni), and nickel plus silymarin (Ni+Sil). Nickel as nickel sulfate (NiSO4 6H20) (20 mg/kg b.wt.) was given intraperitoneally on alternate days until the tenth dose, methanolic extract of milk thistle (100 mg/kg) was given orally to rats for 21 days. The administration of nickel caused a significant decrease in body weight with a significant increase in liver weight. Nickel treatment also produced oxidative liver injury characterized by an increase in serum glucose concentration, levels of hepatic markers enzymes (GPT, GOT, LDH and ALP) and bilirubin. Furthermore, the exposure to nickel significantly increased malondialdehyde level and decreased reduced glutathione concentration and superoxide dismutase, catalase and glutathione peroxidase activities. These results are substantiated with marked changes in the histopathology. However, the treatment with silymarin significantly lowered the level of lipid peroxidation and enhanced the antioxidant status, and resulted a reduction of the necrotic damage caused by nickel and thereby restored the previous biochemical parameters. The results of this investigation showed that nickel induced hepatotoxicity due to the excess generation of free radicals and impairment antioxidant defenses, but the use of silymarin extract countered the adverse effects of nickel to a major extent, suggesting their anti-oxidant and immunity powerful.
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    Protective role of L-cysteine against nickel induced hepatotoxicity in Albino Wistar rats
    (J Pharmacol Med Chem, 2018-11-30) Samira Bouhalit
    OBJECTIVE: The present study was undertaken to investigate the hepatoprotective effect of L-cysteine on nickel-induced oxidative stress in experimental rats. METHODS: Male albino (Wistar) rats were divided into four groups of seven each: the first group was used as controls. The second group was given orally L-cysteine at dose of 100 mg/kg b.wt. The third group was administrated intraperitoneally with nickel sulfate at dose of 20 mg/kg and the fourth one given both L-cysteine and nickel for three consecutive weeks. Liver function markers alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH), total bilirubin and proteins in serum and hepatic malondialdehyde (MDA) and antioxidants parameters including glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) were measured. RESULTS: Nickel treatment produced oxidative liver injury characterized by an increase in hepatic markers enzymes (ALT, AST, ALP, LDH) activities and bilirubin level with a reduction in total proteins concentration. Simultaneously, it led to an increase of MDA level with a reduction of GSH concentration, SOD, CAT and GSH-Px activities. These results are also substantiated with obviously changes in hepatohistology. However, the treatment with L-cysteine significantly ameliorated the previous parameters and resulted in an improvement of the histopathological hepatic lesions. CONCLUSION: Depending on the findings, it can be concluded that L-cysteine possesses a potential antioxidant power effect against nickel hepatotoxicity.