Browsing by Author "Oumaima NAILI"

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    Effect of Abies numidica Extracts on Performance, Blood Parameters and Caecal Microflora of Broiler Chicks.
    (Research Journal of Pharmaceutical, Biological and Chemical Sciences, 2015-12) Oumaima NAILI
    The aim of this study was to investigate the effects of hydroalcoholic and aqueous extracts of Abies numidica on performance, caecal microflora and some blood parameters. Fifty, one-day-old mixed sex broiler chicks were used in the experiment. The animals were allocated into five dietary treatments groups in a complete randomized design and fed for 21 days. The experimental groups were as follow: the control group (CG)-basal diet without plant extract addition, the first experimental group (EG1) with the addition of 2 g of aqueous extract per 1 kg of basal diet, followed by the second experimental group (EG2) 4 g of aqueous extract kg-1 and we used basal diet with the addition of 2 and 4g kg-1 of hydroalcoholic extract in the third (EG3) and the fourth (EG4) experimental groups respectively. The results showed that body weight gain in the first week was significantly increased when the diets were supplemented with 4g hydroalcoholic extract. Feed intake and feed conversion ratio were not affected in birds fed diets with supplements in comparison with the control group. Glucose and cholesterol concentrations were not affected by different treatments but triglyceride and total protein concentrations were significantly increased. In addition, inclusion of 2 levels of both extracts had no effect on Lactic acid bacteria count, Enterobacteriaceae and total aerobes counts. These findings suggest that more studies are needed to investigate beneficial effects of Abies numidica extracts in broiler production.
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    In vitro Antioxidant, anti-Alzheimer and Antibacterial Activities of Ethyl acetate and n-Butanol Fractions of Punica granatum Peel from Algeria
    (Tropical Journal of Natural Product Research, 2023-08-01) Oumaima NAILI
    Abstract Plants constitute a potential source of high-value compounds which can provide a new source of bioactive agents. The present study was carried out to investigate the content of phenolic compounds, the antioxidant, anticholinesterase and antibacterial activities of the peels of pomegranate collected in eastern Algeria. The hydro-ethanolic extract of pomegranate peels was fractionated, then the ethyl acetate and n-butanol fractions were evaluated for their biological activities. DPPH, ABTS, CUPRAC, phenanthroline and reducing power assays were used to evaluate the antioxidant capacity. The enzyme inhibitory activity of the fractions was evaluated against key enzymes in Alzheimer's disease: acetylcholinesterase and butyrylcholinesterase. Moreover, the antibacterial activity of fractions was tested against four bacterial strains: Escherichia coli (ATCC 25922), Bacillus cereus (ATCC 10876), Pseudomonas aeruginosa (ATCC 27853) and Staphylococcus aureus (ATCC 25923). The results demonstrated that the fractions were a good source of phenolics, flavonoids, flavonols and tannins. Furthermore, the extracts showed the highest antioxidant activity than the standards. The extracts exhibited an interesting inhibitory effect against the tested enzymes. The ethyl acetate fraction showed the highest activity against acetylcholinesterase (IC50: 76.51±3.32 μg/mL) while the n-butanol fraction showed the highest activity against butyrylcholinesterase (IC50: 119.15±5.87 μg/mL). Furthermore, the fractions showed promising antibacterial effects against Staphylococcus aureus, Bacillus cereus and Pseudomonas aeruginosa. The results showed that pomegranate peels could be considered a good source of bioactive compounds with potent scavenging activity and reducing ability. In addition, pomegranate peels showed great potential as antibacterial and antineurodegenerative agents.
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    Neuroprotective and Antioxidant Activities of Algerian Punica granatum L. Peel Extracts
    (eurekaselect, 2023-07-31) Oumaima NAILI
    Background: Oxidative stress is implicated in many neurodegenerative disorders, such as Alzheimer’s disease. As an alternative, Punica granatum peels are widely used in the treatment of several ailments. To date, this is the first study on the anti-acetyl/butyrylcholinesterase effects of diethyl ether extract from Algerian pomegranate peels. Objective: This research aimed to evaluate the content of total phenolics, flavonoids, flavonols, and tannins in Punica granatum peel crude extract and its fraction diethyl ether, as well as to assess its antioxidant and neuroprotective activities. Methods: The total phenolic, flavonoid, flavonol, and tannin contents in the extracts were evaluated using spectrophotometric methods. The inhibitory effects on acetyl/butyrylcholinesterase were assessed using the in vitro models. In addition, several methods were used to investigate the antioxidant capacity of extracts, including 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline- 6-sulfonate) (ABTS) free radicals scavenging, phenanthroline, reducing power and cupric ion reducing antioxidant capacity (CUPRAC) assays. Results: Phytochemical analysis revealed that the diethyl ether extract recorded the highest content of total phenolics, flavonoids, flavonols, and tannins with values of 643.3 ± 9.96 μgGAE/mg, 78.97 ± 9.97 μgQE/mg, 31.32 ± 1.79 μgQE/mg and 96.6 ± 13.65 μgTAE/mg, respectively. Moreover, the diethyl ether extract exhibited the strongest activity in the DPPH scavenging assay (IC50=3.36 ± 0.11 μg/mL), ABTS scavenging assay (IC50=1.46 ± 0.10 μg/mL), CUPRAC assay (CA0.5=3.62 ± 0.36 μg/mL), phenanthroline assay (CA0.5=1.04 ± 0.10 μg/mL), reducing power assay (CA0.5=4.14 ± 0.32 μg/mL), antiacetylcholinesterase assay (IC50=114.86 ± 13.66μg/mL), and anti-butyrylcholinesterase assay (IC50=147.00 ± 10.41 μg/mL). Conclusion: The present study has shown the extracts from Punica granatum peel to be a rich source of bioactive compounds, potent antioxidants, and cholinesterase inhibitors. They could be considered as natural agents that can be used in anti-neurodegenerative pharmaceutical formulations
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    Phosphate removing using bacteria isolated from activated sludge for wastewater remediation
    (Journal of Biodiversity and Environmental Sciences, 2015) Oumaima NAILI
    Abstract The objective of the current study is to isolate phosphate removing microbes from activated sludge for wastewater remediation in the area of Khenchela (Eastern Algeria) . Phosphate rate was determined using colorimetric method and batch tests were developed to evaluate the biomass composition of the sludge. Four efficient pure strains isolated from activated sludge samples and identified as Acinetobacter junii, Pseudomonas aeruginosa, Moraxella lacunata, Alcaligenes denitrificans were tested for their capacities of phosphate reduction. The best rate of phosphate removal by pure cultures is observed with Acinetobacterjunii, 76%, followed by Alcaligenes denitrificans, 70.42%, Pseudomonas aeruginosa, 61.78%, and Moraxella lacunata, 50.6%. Our results show that applying of mixed bacterial culture containing mostly isolated strains for bioremediation purpose can be used successfully for the elimination of phosphate from activated sludge of wastewater treatment plants.
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    Phytochemical Screening and Effect of Three Plants Collected from the Region of Khenchela, Algeria against Multi-drug Resistant Pathogenic Bacteria
    (Indian Journal of Novel Drug Delivery, 2022-03-27) Oumaima NAILI
    This work aims to perform phytochemical analysis of hydroethanolic extracts of three medicinal plants commonly used in folk medicine (Juniperus oxycedrus, Eucalyptus globulus, and Matricaria chamomilla) and to evaluate their antibacterial potential against multi-drug resistant bacteria. Disc diffusion method has been used to determine the antibacterial activities and minimum inhibitory concentrations (MIC) of different plant extracts against four Gram negative bacteria (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Enterobacter aerogenes) and two Gram positive bacteria (Staphylococcus aureus and Bacillus subtilis). Antibiotic sensitivity test was performed in Mueller Hinton agar with three commonly used antibiotics (tetracycline, vancomycin and oxacillin). Phytochemical analysis revealed the presence of tannins and free quinones in all the extracts, whereas alkaloids were absent. The results of antibiogram revealed that all strains were resistant to tested antibiotics with exception of Staphylococcus aureus that was found to be susceptible to oxacillin. Also, obtained results showed that hydroethanolic extracts exhibited dosedependent growth inhibitory effects against Staphylococcus aureus and Bacillus subtilis, while no inhibitory effect on Gram negative bacteria was observed. In conclusion, the present work provides useful baseline information for the potential use of the studied plants in the fight against MDR strains.
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    Probiotic potential of lactobacillus strains isolated from fresh bee pollen
    (Scholars Research Library, 2016) Oumaima NAILI
    ABSTRACT The present work aimed to evaluate the probiotic potential of lactobacillus strains isolated from fresh bee pollen. Thirty three lactobacillus isolates were screened for antagonistic activity against six pathogenic bacteria. Ten of them were selected identified as lactobacillus plantarum and evaluated for resistance to acidic pH (2 and 3) and 0.3% bile salts, hydrophobicity and autoaggregation ability. Moreover, their safety was verified by testing haemolytic activity on human blood agar and antibiotic resistance. The results showed that all lactobacillus strains were effective against all indicator bacteria; all strains were able to maintain their viability after 3h exposure to pH 3 and 4h in the presence 0.3% of bile salts. While only five could survive with losses in cell viability after 3h exposure to pH 2. Most strains showed a high hydrophobicity and autoaggregation ability. All lactobacillus strains were resistant to ciprofloxacin, tobramycin, nalidixic acid and colistin. 50% of the strains were susceptible to chloramphenicol, Nitroxolin, penicillin G, Cefoxitin, pristinomycin, cefexim and 80% are susceptible tostreptomycin. No haemolysis was observed on blood agar. Five strains of Lactobacillus plantarum were selected as suitable candidates for industrial use.
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    Screening of Bacteria Isolated from Activated Sludges for Phosphate Removal from Wastewater
    (Journal of Applied Environmental and Biological Sciences, 2015) Oumaima NAILI
    ABSTRACT The objective of the current study is to isolate phosphate removing microbes from activated sludge for wastewater remediation in the area of Khenchela (Eastern Algeria) . Phosphate rate was determined using colorimetric method and batch tests were developed to evaluate the biomass composition of the sludge. Four efficient pure strains isolated from activated sludge samples and identified as Acinetobacter junii, Pseudomonas aeruginosa, Moraxella lacunata, Alcaligenes denitrificans. Our results show that applying of mixed bacterial culture containing mostly isolated strains for bioremediation purpose can be used successfully for the elimination of phosphate from activated sludge of wastewater treatment plants.