Browsing by Author "M. A. HAMIDECHI"

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    Article original Incidence du streptocoque du groupe b (sgb) chez les nouveau-nés nés à Constantine (Algérie) et dans deux de ses banlieues. Intérêt de la détermination des sérotypesIncidence of group b streptococcus (gbs) in neonates born in Constantine (Algeria) and two of its suburbs usefullness of serotyping
    (elsevier, 2003-08) M. A. HAMIDECHI
    Aim. – Group B Streptococcus (GBS) is the germ most often responsible for neonatal infections. Several risk factors are described. The objective of this investigation was to demonstrate the effects of age, sex, and place of birth in neonates GBS infection, including serotype distribution. Method. – A 4 years-long multicentric study was carried out in Constantine and 2 of its suburbs, including 8,711 live neonates. Seven hundred and thirty-three suspected infections were recorded and sorted according to postnatal age (≤ 7 days and > 7 days), sex, and place of birth (related to the mothers’ socioprofessional status). Thirty-one cases of GBS infection were confirmed. Results. – GBS was identified in 53 out of 733 suspected cases of infection (i.e.: 7.2% or 1/14 suspected case). The incidence of early GBS infection was greater than late onset (36 vs 17 out of 53 cases). GBS colonization was not related to age. High socioprofessional status decreased the infection risk significantly in neonates. Conclusion. – This study demonstrates that favorable hygiene and the mother’s high socioprofessional status decrease the risk of neonatal GBS infection
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    CONTRIBUTION DU DIAGNOSTIC BIOCHIMIQUE BACTERIEN DANS L’ETABLISSEMENT DES PARENTES PHYLOGENETIQUES : CAS DES Enterobacteriaceae ET Pseudomonas sp
    (Sciences& Technologie, 2011-12) M. A. HAMIDECHI
    Ten bacterial strains were isolated from clinical samples provided by the bacteriology laboratory (Daksi, Constantine). Among the ten strains studied, eight belong to the Enterobacteriaceae and two are among the Pseudomonadaceae. These strains were presumptively identified by their biochemical characteristics, susceptibility or resistance to antibiotics, and total nitrogen measured by the Kjeldahl method. A phylogenetic profile was produced by phenetic methods based on the calculation of genetic distances using the Neighbor Joining algorithm (NJ) and bioinformatics analysis of 16S rRNA sequences to compare the two phylogenies (phenotypic and genotypic). The analysis of topologies obtained showed that the biochemical phenotype has some limitations in bacterial identification and sequence analysis of 16S rRNA is a key tool in bacterial identification across the case.
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    Incidence de Listeria spp. et autres bactéries psychrotrophes dans le lait cru bovin dans le Nord Est Algérien
    (researchgate, 2011-01) M. A. HAMIDECHI
    In this study a total of 104 milk samples collected from 60 dairy cattle in six farms in the North East part of Algeria were examined for the presence of Listeria spp. and other psychrotrophic bacteria. The isolation was accomplished by cold storage of milk samples followed by plating on blood agar added of Cefazoline. The isolates were identified phenotypicaly by studying cultural, morphological, biochemical criteria; and genotypicaly using the 16S rRNA gene. Psychrotrophic bacteria from numerous genera have been isolated, both gram negative “Acinetobacter spp., Aeromonas spp., Pseudomonas spp., Stenotrophomonas spp., Proteus spp., Yersinia spp., Klebsiella spp., Alcaligenes spp., and Flavobacterium spp.” and gram positive “Enterococcus spp., Listeria spp., Staphylococcus spp., and Bacillus spp.”. Enterococcus spp. was the dominant genera in different seasons. Milk contamination by Listeria spp. was seasonal, its incidence was positive in winter and in spring, however it was absent in summer and autumn.