Browsing by Author "Kheddouma Asma"

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    Effects of the methane-inhibitors Nitrophenol, 5-Nitrobenzimidazol and two new synthetic nitrocompounds on in vitro ruminal fermentation
    (elsevier, 2018-03-11) Kheddouma Asma
    The objective of this study was to examine the effects of four nitrocompounds (Nitrophenol, 5-Nitrobenzimidazol and two synthetic nitrocompounds ABLE 244 and ABLE 245) on methane production and fermentation characteristics using in vitro rumen batch culture. 0, 2, 8 or 12 μM of each nitrocompound were incubated. The higher concentrations of Nitrophenol and 5-Nitrobenzimidazol produced 60% less CH4 (P < 0.05) compared to controls, while two synthetic nitrocompounds ABLE 244 and ABLE 245 had no effect on CH4 production. Quantification of fermentation end-products indicated that fermentation efficiencies were not compromised by the nitro-treatments.
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    Evaluation De Quelques Activités Biologiques Des Extraits De Capparis Spinosa L. Collectée De La Région Des Aurès (Djellal
    (Université Abbes Laghrour -Khenchela-, 2023) Encadreur: Khabtane Amira; Benabbes Ferial; Kheddouma Asma
    Abstract Evaluation of some biological activities of extracts from Capparis spinosa L. collected from the Aurès region (Djellal). The main objective of this work is to evaluate several biological activities (antibacterial, antifungal, and antioxidant) of crude organic extracts (petroleum ether, chloroform, methanol) and a non-organic extract (aqueous) obtained from the leaves and stems of Capparis spinosa collected from the Djellal region, wilaya Khenchela, using the solid-liquid extraction procedure with different solvents of varying polarities. These extracts were subjected to phytochemical screening, and preliminary tests revealed the presence of cardiac glycosides, mucilages, essential oils, and tannins, as well as the absence of coumarin. The evaluation of antibacterial activity demonstrated that C. spinosa extracts exhibited significant antibacterial activity against certain bacterial strains (Staphylococcus aureus, Bacillus cereus, Pseudomonas fluorescens, Escherichia coli), highlighting their potential as antimicrobial agents. Regarding antioxidant activity against the DPPH radical using a spectrophotometer, Capparis spinosa extracts showed a high capacity to neutralize free radicals, indicating their potential as protective agents against oxidative stress. Furthermore, tests were conducted using the disc diffusion method on PDA agar medium with two fungi, Aspergillus niger and Penicillium spp. These extracts also exhibited antifungal activity, suggesting their potential use in the treatment of fungal infections. Therefore, Capparis spinosa extracts demonstrate promising activities as antibacterial, antifungal, and antioxidant agents, thus paving the way for their potential use in the medical and pharmaceutical fields. تقييم بعض الانشطة الحيوية لمستخلصات Capparis spinosa L قطفت من منطقة الأوراس (جلال) الهدف من هذا العمل هو تقييم بعض الأنشطة البيولوجية مضادة للبكتيريا، مضادة للفطريات ومضادة للأكسدة) للمستخلصات العضوية الخام الاثير البترولي، كلوروفورميك ميثانول) ومستخلص غير عضوي (ماني) المستخلصة من أوراق وسيقان نبات الكبار التي قطفت من منطقة جلال، ولاية خنشلة، باستخدام إجراء الاستخلاص الصلب - السائل بواسطة مذيبات ذات قطبيات مختلفة. تم تعريض هذه المستخلصات لاختبارات الفحص النباتي الكيميائي، وأظهرت هذه الاختبارات الأولية وجود الجليكوزيدات، القلبية، المخاطين، الزيوت العطرية والتانينات، وعدم وجود الكومارين و مشتقات الانثرسينيك. اظهر تقييم النشاط المضاد للبكتيريا أن مستخلصات الكبار تظهر نشاطا ملحوظا ضد بعض من سلالات البكتيريا (Staphylococcus aureus, Bacillus cereus, Pseudomonas fluorescen, Escherichia coli) مما يسلط الضوء على نبات الكبار على انه عامل مضاد للبكتريا. باستخدام الطيفي المرني، أظهرت مستخلصات نبات الكبار فيما يتعلق بالنشاط المضاد للأكسدة اتجاه الجذر الحر DPPH قدرة مرتفعة على إرجاع الجذور الحرة، مما يشير إلى إمكانية استخدامها كعامل حماية ضد التوتر التاكسدي. بالإضافة إلى ذلك، في وسط جيلوز (PDA) باستعمال طريقة القرص اجريت الاختبارات مع نوعين من الفطريات، Aspergillus niger et Penicillium spp، المستخلصات أيضا أظهرت نشاط مضادا للفطريات، مما يشير إلى أنه يمكن استخدامها في علاج الالتهابات الفطرية، لذلك تقدم مستخلصات Capparis spinosa أنشطة واعدة كعوامل مضادة للبكتريا، مضادة للفطريات ومضادة للأكسدة، وبالتالي تمهد الطريق في استخدمها في المجالات الطبية والصيدلانية.
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    In vitro antimicrobial activity of Salvadora persica and Juglans regia extracts against microbial strains from oral cavity
    (elsevier, 2021) Kheddouma Asma
    used for the oral hygiene in Arabic countries. Four extracts prepared with organic solvents of increasing polarity; petroleum ether, chloroform, ethyl acetate, and methanol were used to test the antibacterial and antifungal activities of these plants with three different concentrations of each extract; 5, 10 and 15mg/ml. The results showed that Juglans regia seems to be more efficient against the isolated strains than Salvadora persica. Moreover the ethyl acetate extract of J. regia showed a high degree of antimicrobial activity. Forty bacterial isolates were identified from the oral cavity of healthy individuals of different age categories (from 10 to 44 years) and only seven isolates were selected and characterized as Staphylococcus saprophyticus (B1), Proteus vulgaris (B2), Pseudomonas aeruginosa (B3), Staphylococcus aureus (B4), Serratia marcescens (B5), Streptococcus salivarius (B6), Streptococcus mutans (B7) and a yeast species identified as Candida albicans (Y). The highest efficiency of all extracts of the two plants achieved with Streptococcus mutans (mean of diameters of inhibition zones MDIZs = 10.29mm) followed by Streptococcus salivarius (MDIZs = 9.75mm), Candida albicans (MDIZs = 6.45mm), Proteus vulgaris (MDIZs = 5.75mm), Staphylococcus aureus (MDIZs = 5.62mm), Serratia marcescens (MDIZs = 5.58mm), Pseudomonas aeruginosa (MDIZs = 4.87mm) and Staphylococcus saprophyticus (MDIZs = 3.08mm).