DSpace Repository :: Browsing by Author "Kenza Kadi"

Browsing by Author "Kenza Kadi"

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Biological activities and phenolic compounds of olive oil mill wastewater from Abani, endemic Algerian variety
(Nature, 2022) Dalila Addad; Kenza Kadi
The current study aimed to determination of cytotoxicity, antioxidant, anti-inflammatory, anti- hemolytic, and anticoagulant activities of phenolic compounds extracted from olive oil mill wastewater (OMW) issue from the cold extraction of olive oil from Khenchela eastern in Algeria. The LC-MS (liquid chromatography-mass spectrometry) results were revealed the presence of 20 phenolic compounds in the extract of OMW and mostly consisted of Kaempferol, 4,5-di-O-caffeoyquinic acid, quinic acid, and caffeic acid. The extracts possessed effective reducing power (FRAP) and high radical scavenging activity against DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS + (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) free radicals, and it inhibited cytochrome c reduction in a dose-dependent manner. They exert a protective effect on red blood cells, and they were found to exhibit the highest inhibitory effect anti-inflammatory activity using inhibition of protein denaturation (IPD) and membrane stabilizing potential (MSP) tests (80.46±3.81 μg/mL and 87.43 ± 0.66 μg/mL) more than the standard used. The extract also showed the greatest anticoagulant activity in both the endogenous and exogenous routes (44.77 ± 0.25 s and 15.84 ± 0.12 s, respectively). Based on these findings, it is reasonable to infer that OMW is a good source of natural phenolic compounds with potential antioxidant, anti-inflammatory, and anticoagulant properties.
DETERMINATION OF PHENOLIC COMPOUNDS AND ANTIOXIDANT ACTIVITY OF WHITE HENBANE (HYOSCYAMUS ALBUS L.) PLANT TREATED BY KINETIN (K)AND 2,4-DICHLOROPHENOXYACETIC ACID (2,4-D) IN MILA, ALGERIA
(Journal of Fundamental and Applied Sciences, 2018-09-01) Kenza Kadi; D. Dib; S. Hamli; D. Addad
The present work deals with the evaluation of the antioxidant activity of the extracts of the white henbane (Hyoscyamus albus L.) of the solanaceae family, treated with phytohormones 2,4-Dichlorophenoxyacetic acid (2, 4-D) andKinetin (K)in doses 0, 10,20mg / l), widely used in traditional medicine and very rich in alkaloids. Preliminary phytochemical screening showed that treatment with phytohormones had no effect on the quality of the secondary metabolites. The results of the phytochemical study of the plant showed that the treatment with 2,4-D and K had a significant effect by reduction compared to the untreated plant on the phenolic compounds studied. The evaluation of the antioxidant activity in vitro was investigated by the DPPH trapping method and showed that the treatment with 2,4-D and K had a significant effect on the antioxidant activity so all the extracts of the various treatments of H. albus can act as radical scavengers.
Diferent indole‑3‑acetic acid and 6 benzyl amino purine concentrations afect biomass, phenolic proile, and bioactivity in Mentha rotundifolia L.
(Journal of Food Measurement and Characterization, 2023-06-06) Kenza Kadi; Dalila Addad
Although the use of phytohormones for crop improvement has great potential, little is known about the molecular efects of phytohormones in crops. This work investigated the efect of the exogenous application of plant growth regulators (PGRs), indole-3-acetic acid (IAA), and cytokinin-like 6-benzyl amino purine (BAP) on plant biomass and the phytochemical and biological parameters of Mentha rotundifolia L. aerial parts and roots. The results showed that the application of IAA and BAP signiicantly inluenced the accumulation of phenolic compounds in the plant organs. Treatment with PGRs also induced remarkable increases in the amounts of individual phenolic compounds, with the greatest increases observed for salvianolic and rosmarinic acids in the aerial parts treated with 20 or 10 mg/mL of BAP. Salvianolic acid also showed the most signiicant increase in the roots of plants treated at 10 mg/mL (from 18.232 to 41.317 µg/g of extract). Furthermore, enhanced antioxidant and inhibitory enzyme efects in the treated plants was observed. Our indings suggest that exogenous hormones could be used to improve the synthesis of phenolic compounds and, as a result, the bioactivity of medicinal or food plants.
EFFECT OF EARTHWORM CASTS FROM A NATURAL MEADOW ON SOME SOIL PHYSICO-CHEMICAL CHARACTERISTICS AND LETTUCE GROWTH
(Journal of Fundamental and Applied Sciences, 2020-09-01) Dalila Addad; Kenza Kadi; Sofia Hamli; Dounia Dib
Fertile Grounds are the source for agricultural production and effective nutrients management. We aim at surveying the contribution effect of earthworm casts on physical and chemical soil characteristics (pH, organic matter, electrical conductivity, exchangeable cations) and their impact on morphophysiological parameters of lettuce. Basically, for this operation we have choose three levels of intake (100, 250 and 500g of earthworm casts/pot). The obtained results have shown that the earthworm casts are rich in nutrients such as nitrogen, calcium, potassium and organic matter. Correspondingly, the contribution of earthworm casts has increased the soil content of OM, NTK, K+ and CaCO3. Also, it has a positive effect on chlorophyll, aerial, root biomass and on lettuce growth in general. The results also have indicated that the 500g dose of earthworm casts/pot have been increased the various parameters.
Effect of storage time on the biodegradability of olive oil mill wastewater from the cold extraction of olive oil system
(The EuroBiotech Journal, 2021) Kenza Kadi; Dalila Addad
The olive oil processing industry’s liquid effluents (OMW) have a polluting capacity for the ecosystems. This study aimed to evaluate the physicochemical quality of OMW of two varieties of separated and combined olives cultivated in Khenchela Eastern Algeria, from the cold extraction of the olive oil extraction system. These was to determine their degree of pollution and biodegradability during one year of storage at ambient temperature to recommend the correct treatment for each storage time. Results of the measured parameters pH, EC,TSS percent, H 2 O percent, lipids, DM, OM, MM, VM, COT percent, NTK percent, C/N, BOD 5 , COD, BI, TOM, BOD 5 /COD show that wastewater from olive oil mills has an acid pH, and they are very loaded with organic matter evaluated in terms of COD and BOD 5 , quite filled with minerals. The storage of olive mill waste can reduce progressively the pollution caused by this waste. Whereas during one year, the reduction rate of COD, BOD 5 , TOM, BI, is respectively 29.4%, 54.8%, 39.16%, 54.2%, but C/N, BOD 5 /COD continue to increase as well as pH that continues to decrease during the storage. Accordingly, storing olive mill waste during a year reduces its pollution rate, so it is slowly biodegradable. When disposing of it, an adequate treatment procedure must be required to protect the environment.
EVALUATION OF THE ANTIBACTERIAL AND ANTICOGULANT ACTIVITIES OF Linum usitatissimum L.EXTRACTS EVALUATION OF THE ANTIBACTERIAL AND ANTICOGULANT ACTIVITIES OF Linum usitatissimum L.EXTRACTS EVALUATION OF THE ANTIBACTERIAL AND ANTICOGULANT ACTIVITIES OF Linum usitatissimum L.EXTRACTS
(Journal of Fundamental and Applied Sciences, 2020) Kenza Kadi
The objective of this study is to evaluate the phytochemical constituents and anticoagulant, and antibacterial activities of phenolic extracts obtained from flaxseed. The quantitative estimation of total phenols by the colorimetric method showed that both aqueous and methanolic extracts are low in these compounds. The evaluation of the antibacterial activity of polyphenols was carried out by the disc method by determining the minimum inhibitory concentration (MIC). The results showed that no antibacterial activity with polyphenols was observed; against the five bacteria tested.The anticoagulant activity of polyphenols was also evaluated in vitro using the cephaline-kaolin (TCK) and Quick time (TQ) tests. The coagulation times obtained on normal plasma indicate that they have moderate activity on both coagulation pathways. The objective of this study is to evaluate the phytochemical constituents and anticoagulant, and antibacterial activities of phenolic extracts obtained from flaxseed. The quantitative estimation of total phenols by the colorimetric method showed that both aqueous and methanolic extracts are low in these compounds. The evaluation of the antibacterial activity of polyphenols was carried out by the disc method by determining the minimum inhibitory concentration (MIC). The results showed that no antibacterial activity with polyphenols was observed; against the five bacteria tested.The anticoagulant activity of polyphenols was also evaluated in vitro using the cephaline-kaolin (TCK) and Quick time (TQ) tests. The coagulation times obtained on normal plasma indicate that they have moderate activity on both coagulation pathways. The objective of this study is to evaluate the phytochemical constituents and anticoagulant, and antibacterial activities of phenolic extracts obtained from flaxseed. The quantitative estimation of total phenols by the colorimetric method showed that both aqueous and methanolic extracts are low in these compounds. The evaluation of the antibacterial activity of polyphenols was carried out by the disc method by determining the minimum inhibitory concentration (MIC). The results showed that no antibacterial activity with polyphenols was observed; against the five bacteria tested.The anticoagulant activity of polyphenols was also evaluated in vitro using the cephaline-kaolin (TCK) and Quick time (TQ) tests. The coagulation times obtained on normal plasma indicate that they have moderate activity on both coagulation pathways.
EVALUATION OF THE ANTICOAGULANT ACTIVITY OF MARGINS FROM OLIVES EXTRACTION IN THE KHENCHELA REGION
(Journal of Fundamental and Applied Sciences, 2020) Kenza Kadi; S. Hamli; D. Dib; D. Addad
The objective of this study is the evaluation of the anticoagulant activity of polyphenols obtained from olive extracts (Olea europaea L.) Of the margines of two varieties cultivated at Khenchela (East of Algeria) , the first one is souidi from Chacher and the second one is chemlal from Baghai. The physicochemical characteristics of the two varieties studied showed a difference in water content, total solid content (dry matter), total solid in suspension, potential hydrogen, and especially its content in polyphenols and flavonoids. The quantitative analysis of the two extracts has revealed a great richness of the margines of the souidi variety of polyphenols and flavonoids content compared to the margines of the chemlal variety. The dosage of polyphenols is 0.8 mg EAG / mlE) for Souidi and (0.5 mgEAG / mlE) for Chemlal. The in vitro evaluation of the anticoagulant activity of the margines of two varieties souidi and chemlal revealed that the margines of the souidi variety has a good anticoagulant activity vis-à-vis the endogenous pathway Time of Céphaline Kaolin (TCK).
Experimental research on water chemistry evolution in case of inadequate conservation protocols: application on surface and groundwater
(Applied Water Science, 2021) Kenza Kadi; Dounia Dib; Nawel Ababsa; Dalila Addad
The objective of this study conducted from October to December 2020 in the Natural Resources and Sensitive Environment Development Laboratory was to demonstrate the importance of conservation protocols respect throw evolution of water chemistry. Ninety-nine samples representing three water types (tap, spring and mineral water) were analyzed. Storage was at laboratory temperature with no conservation protocol. Studied parameters (temperature, pH, CE, dissolved oxygen, oxydoreduction potential, total dissolved solids, HCO 3 − , Ca 2+ , Mg 2+ , SO 4 2− and Cl − ) were determined using standard methods. The data registered from physico-chemical parameters were subjected to diferent analytical methods to assess the time afect on their values compared with initial state. The results indicate that pH and alkalinity (exprimed in HCO ) are the most vulnerable to evolution processes with highly signiicant time factor efect, while the concentrations of chlorides and sulfates with conductivity levels are statistically less evolved. PCA analysis accounting 71.43% of the total variance examines contribution of water type composition as a second variation factor. Projection through F1*F2 plan demonstrates clearly two groups with surface waters (tap water) which are excessively mineralized and groundwaters (spring and bottled waters) in which pH and magnesium parameters variations are the best illustrated. 3 −
HPAEC-PAD, biochemical characterization, and evaluation of the antioxidants activities of polysaccharides extracted from Olive Mill Wastewater of two endemic varieties of Khenchela region, Algeria
(journal homepage, 2023) Kenza Kadi; Dalila Addad
Olive Mill Wastewater (OMWW) is considered to be one of the by-products of the extraction of olive oil that causes serious problems to the environment. This study describes a qualitative and quantitative analysis of biochemical characterizations and assessment of polysaccharides antioxidant activities from cold extraction of two endemic varieties from Khenchela region, eastern Algeria. As a result, the physicochemical characteristics of these OMWWs (Chemlal and Ferkani) were found to be acidic (pH: 4.77–5.1) and saline (1.35–1.40 mg/l). According to Chemlal and Ferkani, the dry matter (DM), total suspended solids (TSS) and mineral matter (MM) are respectively (159.3 ± 14.03 g/L, 4.13 ± 0.04 g/L, 10 ± 0.5 g/L and (117.4 ± 12.03 g/L, 1.06 ± 0.1 g/L, 9.7 ± 1 g/L). In addition, the COD and DBO5 requirements are (9625.6 ± 13.42 mg/l; 45 ± 08 mg/l for Chemlal) and (10490.6 ± 23.84 mg/l; 440 ± 3 mg/l for Ferkani) respectively. Due to their high content of organic matter and mineral salts, the OMWW studied have very little potential for biodegradation. Three extracts were prepared for each cultivar: insoluble alcohol fraction (AIR), soluble water fraction (SF) and insoluble water fraction (IF). The biochemical characterisation was carried out by colorimetric assays using an appropriate spectrophotometer for the determination of total sugars (OT), reducing sugars (OR), neutral sugars, protein, nitrogen and phenolic compounds. The qualitative and quantitative analysis of the polysaccharides was carried out using high- performance anion-exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD). The results obtained showed heterogeneous composition of nine monosaccharides for each fraction at different levels. The total dry matter (%DM) of the Chemlal fractions (AIRC, SFC, IFC) are respectively (14.44%, 16.93%, 15.68%) and those of the Ferkani fractions (AIRF, SFF, IFF) are respectively (10%, 15.05%, 10.52%). The antioxidant activities of two soluble fractions (SFC and SFF) were evaluated using five assays: DPPH, ABTS, CUPRAC, phenthroline and hydrogen peroxide, when the best antioxidant activity is provided by hydrogen peroxide.
IDENTIFICATION OF HYDROGEOCHEMICAL PROCESSES IN A NORTH EASTERN ENDOREIC BASIN UNDER SEMI-ARID CONDITIONS (ALGERIA)
(Journal of Fundamental and Applied Sciences, 2017) Kenza Kadi; D. Dib
Statistical and geochemical methods were used to identify the factors influencing the behavior of groundwater in the Tarf watershed, characterized by a high water deficit. The chemical facies deduced from the piper diagram and the correlations between different major cations and anions evoked a dominance of the evaporite minerals with respect to the carbonates which are saturated and precipitated. Besides the projection of the results of a few points followed over a period of 4 years (2011-1015) on the Gibbs plot demonstrates the dominance of the processes of evaporation - precipitation with respect to the erosion which is very slowed during the last years due to the fact of lack of effective precipitation.
In Vitro Evaluation of Antibacterial and Anticoagulant Activities of In Vitro Evaluation of Antibacterial and Anticoagulant Activities of Harmala Alkaloids Roots Harmala Alkaloids Roots
(Journal of Bioresource Management, 2023) Kenza Kadi
Peganum harmala L. is a wild herbal plant rich in active compounds, and is among the most used plants in local medicine. The purpose of this study is to contribute to the evaluation of some biological activities of alkaloids extract of P. harmala roots. Using the classical method of extraction of β-carboline alkaloids, the yield of alkaloids in the roots was estimated at 2.34 %. The thin layer chromatography (TLC) method identified the two components Harmine and Hrmaline in the total alkaloid extract of the roots. We evaluated the antibacterial activity of harmala alkaloids in roots against three referenced bacterial strains, and this using the agar medium diffusion method, all extracts showed significant activity against all tested bacterial strains, the largest inhibition zone diameter of 20 mm was recorded with Staphylococcus aureus at a concentration of 50 mg/ml of crude alkaloids. The anticoagulant activity of alkaloid extracts was also examined using the prothrombin time (PT) and partial activation thromboplastin time (APTT) tests. The clotting times obtained in normal plasma indicate that they have good activity in both coagulation pathways compared with sodium heparin, especially in the prolongation of blood clotting in the intrinsic pathway.
Investigation of Photoprotective, Anti-Inﬂammatory, Antioxidant Capacities and LC–ESI–MS Phenolic Proﬁle of Astragalus gombiformis Pomel
(2021) Kenza Kadi; Nawal Mekersi
Plant-derived compounds have recently been gaining popularity as skincare factors due to their ability to absorb ultraviolet radiations and their anti-inﬂammatory, and antioxidant properties. In this light, this work aimed to evaluate in vitro the pharmacological activities of the butanolic extract prepared from the aerial parts of Astragalus gombiformis Pomel, an endemic species to southern Algeria. The sun protection factor was used to assess the photoprotective effect (SPF), the protein denaturation method to determine the anti-inﬂammatory activity, and brine shrimp nauplii and OxHLIA assay, respectively, to assess the cytotoxicity and antioxidant capacity of A. gombiformis. In addition, LC–ESI–MS analysis was employed for the characterization of the phenolic constituents of A. gombiformis. The results showed that A. gombiformis had high capacity for absorbing UV radiations with an SPF of 37.78 0.85 and signiﬁcant anti-inﬂammatory activity with a percentage inhibition of 75.38% which is close to that of diclofenac and ketoprofen. In addition, A. gombiformis was found to have effective cytotoxicity against Artemia nauplii with a DC50 value of about 44.7 g/mL, but a weak hemolytic effect against human erythrocytes. LC–ESI–MS results detected the presence of 17 phenolic compounds with a predominance of cirsiliol, silymarin, quercitrin (quercetin-3-Orhamnoside), and kaempferol. Taken together, these results suggest that A. gombiformis extract could be used as a skincare agent in cosmetic formulations, providing excellent antioxidant and anti-inﬂammatory protection, allowing the treatment of skin conditions, as well as a pharmaceutical agent with multidimensional applications.
INVOLVEMENT OF ABIOTIC STRESS TOLERANCE MECHANISMS IN BIOTIC STRESS TOLERANCE IN DURUM WHEAT
(Journal of Fundamental and Applied Sciences, 2020) Kenza Kadi; S. Hamli; D. Addad
The objective of our study is to implicate the mechanisms of tolerance to abiotic stress by the synthesis of metabolites in tolerance to biotic stress. The extracted metabolites; proline, sugars and polyphenols from durum wheat seedlings subjected to heat shock (40 °C), used to test antifungal activity on two fungal strains, powdery mildew and penicillium, under controlled conditions. The boussellam variety is more tolerant of applied stress than the Ciccio and Vitron varieties. The concentration of the three osmolytes varies from one variety to another; it increases in genotypes stressed compared to controls. Antifungal activity results in the appearance of an inhibition zone around the disc impregnated with the studied extract. Sugars have proven to be a highly effective antifungal agent compared to proline and polyphenols with maximum values (28,33 ± 2 mm) in oidium and (29 ± 1 mm) in penicillium.
LC/MS-MS Analysis of Phenolic Compounds in Hyoscyamus albus L. Extract: In Vitro Antidiabetic Activity, In Silico Molecular Docking, and In Vivo Investigation against STZ-Induced Diabetic Mice
(See discussions, stats, and author profiles for this publication, 2023) Kenza Kadi
This study aimed to investigate the chemical composition and antidiabetic properties of cultivated Hyoscyamus albus L. The ethanol extract was analyzed using LC-MS/MS, and 18 distinct phenolic compounds were identiﬁed. Among these, p-coumaric acid (6656.8 3.4 g/g), gallic acid (6516 1.7 g/g), luteolin (6251.9 1.3 g/g), apigenin (6209.9 1.1 g/g), and rutin (5213.9 1.3 g/g) were identiﬁed as the most abundant polyphenolic molecules. In the in vitro antidiabetic experiment, the ability of the plant extract to inhibit -glucosidase and -amylase activities was examined. The results indicated that the extract from H. albus L. exhibited a higher inhibitory effect on -amylase compared to -glucosidase, with an IC50 of 146.63 1.1 g/mL and 270.43 1.1 g/mL, respectively. Docking simulations revealed that luteolin, ﬁsetin, and rutin exhibited the most promising inhibitory activity against both enzymes, as indicated by their high contrasting inhibition scores. To further investigate the in vivo antidiabetic effects of H. albus L., an experiment was conducted using STZ-induced diabetic mice. The results demonstrated that the plant extract effectively reduced the levels of cholesterol and triglycerides. These ﬁndings suggest that H. albus L. may have therapeutic potential for managing hyperlipidemia, a common complication associated with diabetes. This highlights its potential as a natural remedy for diabetes and related conditions.
Maceration and Liquid-liquid extractions of phenolic compounds and antioxidants from Algerian olive oil mill wastewater
(Research Article, 2022-04-13) Kenza Kadi; Dalila Addad
Olive oil mill wastewater (OMW) is a major waste stream from the olive oil industry. It is highly polluted due to phenolic compounds. The present study focused on the physicochemical properties of OMW as well as the quantitative and qualitative effects of two methods of extraction (maceration and liquid-liquid extraction) of phenolic compounds.
Phenolic compounds and biological activities of phenolic extract of olive oil mill wastewater issue from the cold extraction of olive oil from Khenchela (Algeria)
(Research Article, 2021-04-12) Kenza Kadi; Dalila Addad
This study aimed to the determination of antioxidant, anti-inammatory, and anticoagulant activities of phenolic compounds present in olive oil mill wastewater (OMW) issue from the cold extraction of olive oil from Khenchela eastern of Algeria.After polyphenols extraction, a quantity and quality analysis by LC-MS was made. The LC-MS (liquid chromatography–mass spectrometry)results were revealed the presence of 20 phenolic compounds in theextract of OMW which were: (quinic acid, gallic acid, protocatechuic acid, caffeic acid, p-coumaric acid, rutin, transfrolic acid, hyperoside (quercetin-3-o-galactoside), luteolin-7-oglucoside, naringin, 4.5-di-caffeoyquinic acid, quercetrin(quercetin-3-o-rhamonosid), apegenin-7-oglucoside, salviolinic acid, kampherol, quercetin, naringenin, apegenin,cirsiliol,andcirsilineol).TheresultsofantioxidantactivitywithDPPH(2,2-diphenyl-1-picrylhydrazyl),ABTS+(2,2'-azino-bis(3ethylbenzothiazoline-6-sulfonicacid),andFRAP(ferricreducingabilityofplasma)showed that theIC(half-maximal inhibitory concentration) were(9.62 ± 0.28µg/mL, 7.10 ± 0.11µg/mL and 3.59 ± 0.24µg/mL)respectively. The extract of OMW was found to exhibit the highestinhibitory effectanti- 50 inammatory activity usinginhibition of protein denaturation(IPD) and membrane stabilizing potential (MSP) tests (80.46 ± 3.81; 87.43 ± 0.66 µg/mL) more than the standard used. In addition, the extract had the best anticoagulation activity in the endogenous and exogenous pathways (44.77 ± 0.25s; 15.84 ± 0.12s). Based on these results, it is right to conclude that OMW is an important source of natural phenolic compounds that have important antioxidant, anti-inammatory, and anticoagulant activities.
Physicochemical Characterization and Estimation of the Pollution Degree of Olive Oil Mill Wastewaters from the Cold Extraction System and the Traditional System
(2023) Kenza Kadi
live oil production is widespread in Mediterranean countries such as Algeria. One of the main by-products from olive oil extraction is olive oil mill wastewater (OMW). It considers as an environmental problem for the producing countries. This study aimed to compare the physicochemical characterization and the pollution degree of OMW obtained from two different extraction systems, cold extraction system and traditional system, in Khenchela, eastern Algeria. The results of the physicochemical analyzes have shown that olive mill wastewaters from the two systems were very acidic and very rich in organic and mineral matter. There was a signiﬁcant effect on electrical conductivity (EC), biological oxygen demand (BOD ), chemical oxygen demand (COD), fatty matter (FM), organic matter (OM), and polyphenols (PP). While there was no signiﬁcant difference for the parameters pH, humidity (H%), dry matter (DM), total suspended solids (TSS%), and mineral matter (MM). For the two pollution indices studied, BOD 5 /COD and BI (biodegradability index), there was not a signiﬁcant effect. It was recorded approximately similar values. In conclusion, the cold extraction system was the least polluting compared to the traditional extraction system. In addition, the pollution indicators clearly demonstrated the biodegradable nature of these wastewaters, for which biological remediation is appropriate.
Phytochemical Screening and Assessment of The Antioxidant Activity of Bio-phenols of Olive Oil Mill Wastewater From The Cold Extraction of Olive Oil in Khenchela Region Eastern of Algeria
(Journal of Renewable Energies ICREATA’21 Adrar, 2021) Kenza Kadi
The objective of this study was to evaluate in vitro the antioxidant activity of polyphenols from olive oil mill wastewater in Khenchela region eastern of Algeria after conducting a phytochemical screening. The extraction of polyphenols was done by the maceration method. The quantitative estimate of the total polyphenols and flavonoids was evaluated by the colorimetric method. Antioxidant activity was evaluated in vitro using three tests DPPH, ABTS and FRAP and ascorbic acid as a reference antioxidant. The results obtained showed that the phytochemical screening carried out allowed us to highlight the presence of polyphenols, flavonoids, tannins and reducing sugars with large quantities, sterols and terpenoids in lesser amounts. The values of total polyphenols and flavonoids were sequential: (925.8 ± 8.1 µg GAE/mL) and (45.97 ± 9.5 µg QE /mL). The values of the antioxidant activity obtained from the three tests DPPH, ABTS, and FRAP were successively the followings: (IC 50: 144.52 ± 1 µg/mL), (IC 50 : 169.6 ± 35.3 µg/mL) and (IC 50 : 248.13 ± 10.1 µg/mL). At the end of this study, it appears that OMW polyphenolic extract has an important antioxidant power.
Phytochemical Screening and Radical Scavenging Activity of Whole Seed of Durum Wheat (Triticum durum Desf.) and Barley (Hordeum vulgare L.) Varieties
(Jordan Journal of Biological Sciences, 2017-09-04) Sofia Hamli; Kenza Kadi; Dalila Addad
Three durum wheat (Triticum turgidum L. var. durum) cultivars, namely Bousselam, Vitron, and Gaviota durum, and one barley genotype (Hordum vulgare L.), Fouara, grown under semi-arid conditions were compared for their total phenolic and flavonoids content and antioxidant activities. Antioxidant activity was tested using DPPH radical scavenging assay method. The phytochemical screening revealed the presence of tannins, flavonoids, coumarins, saponins and phenolic compounds in each variety seeds. The results of the present study indicate significant differences among the evaluated varieties in terms of total phenolic and flavonoid contents and for radical scavenging capacity. Among the tested varieties Gaviota durum showed high total phenolic (95.32± 0.27 μg /mg) and flavonoid content (78.80 ± 0.27 μg/mg) and an intermediate radical scavenging capacity. While barley variety Fouara expressed high radical scavenging capacity (54.8% ±0.34) and intermediate total phenol and flavonoids contents. The tested durum wheat and barley varieties possessed varying but meaningful antioxidant activities which were not significantly correlated to their phenol and flavonoid contents. It is necessary to ensure that increased bioactive components in grains are combined with good agronomic performance, high grain yield and high quality for processing. The results of the present study should have significant implications for plant breeders as well as for grain and food processors.
Therapeutic potential of Hyoscyamus niger-derived compounds: Targeting ovarian cancer through antioxidant activity and EGFR tyrosine kinase inhibition
(Journal of King Saud University - Science, 2024) Kenza Kadi
Ovarian cancer poses a significant challenge to women’s health, necessitating innovative therapeutic approaches. This study investigates the therapeutic potential of compounds derived from Hyoscyamus niger, a medicinal plant with a history of traditional use, focusing on their antioxidant properties and their ability to inhibit epidermal growth factor-receptor (EGFR) tyrosine kinase a promising target in cancer therapy. LC-ESI-MS/MS analysis of H. niger extract revealed the presence of 21 phenolic compounds, with luteolin and p-coumaric acid notably exhibiting the highest concentrations. The extract displayed robust antioxidant activity across multiple assays, and its performance in the Reducing Power assay highlights its potential in reducing oxidative stress. Molecular docking results indicate that several phenolic compounds may serve as EGFR inhibitors, offering promising avenues for further investigation in cancer therapy. Notably, hyperoside emerged as a strong candidate due to its favorable binding energy and interactions within the EGFR active site. This study demonstrates the therapeutic promise of H. niger-derived compounds in targeting ovarian cancer through antioxidant activity and EGFR inhibition. Further research is warranted to validate their efficacy and safety, potentially opening up new avenues for ovarian cancer treatment.

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