Browsing by Author "Dalila Addad"

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    Biological activities and phenolic compounds of olive oil mill wastewater from Abani, endemic Algerian variety
    (Nature, 2022) Dalila Addad; Kenza Kadi
    The current study aimed to determination of cytotoxicity, antioxidant, anti-inflammatory, anti- hemolytic, and anticoagulant activities of phenolic compounds extracted from olive oil mill wastewater (OMW) issue from the cold extraction of olive oil from Khenchela eastern in Algeria. The LC-MS (liquid chromatography-mass spectrometry) results were revealed the presence of 20 phenolic compounds in the extract of OMW and mostly consisted of Kaempferol, 4,5-di-O-caffeoyquinic acid, quinic acid, and caffeic acid. The extracts possessed effective reducing power (FRAP) and high radical scavenging activity against DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS + (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) free radicals, and it inhibited cytochrome c reduction in a dose-dependent manner. They exert a protective effect on red blood cells, and they were found to exhibit the highest inhibitory effect anti-inflammatory activity using inhibition of protein denaturation (IPD) and membrane stabilizing potential (MSP) tests (80.46±3.81 μg/mL and 87.43 ± 0.66 μg/mL) more than the standard used. The extract also showed the greatest anticoagulant activity in both the endogenous and exogenous routes (44.77 ± 0.25 s and 15.84 ± 0.12 s, respectively). Based on these findings, it is reasonable to infer that OMW is a good source of natural phenolic compounds with potential antioxidant, anti-inflammatory, and anticoagulant properties.
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    DOES EARTHWORMS DENSITY REALLY MODIFY SOIL'S HYDRODYNAMIC PROPERTIES IN IRRIGATED SYSTEMS WITH RECYCLED WATER?
    (Journal of Fundamental and Applied Sciences, 2016-05-01) Nawal Ababsa; Dalila Addad
    ABSTRACT Our study has the general objective to understand the impact of the valuation of treated water on earthworm abundance and total porosity of the soil and the effect of the interaction between these two physical-biological components of the hydrological functioning of soils. It was carried out on the meadows soils of the valley of Wadi Bousselam. Although the treated water has high organic and particulate filler, it improved the earthworm abundance, total porosity and hydraulic conductivity of the soil.
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    EFFECT OF EARTHWORM CASTS FROM A NATURAL MEADOW ON SOME SOIL PHYSICO-CHEMICAL CHARACTERISTICS AND LETTUCE GROWTH
    (Journal of Fundamental and Applied Sciences, 2020-09-01) Dalila Addad; Kenza Kadi; Sofia Hamli; Dounia Dib
    Fertile Grounds are the source for agricultural production and effective nutrients management. We aim at surveying the contribution effect of earthworm casts on physical and chemical soil characteristics (pH, organic matter, electrical conductivity, exchangeable cations) and their impact on morphophysiological parameters of lettuce. Basically, for this operation we have choose three levels of intake (100, 250 and 500g of earthworm casts/pot). The obtained results have shown that the earthworm casts are rich in nutrients such as nitrogen, calcium, potassium and organic matter. Correspondingly, the contribution of earthworm casts has increased the soil content of OM, NTK, K+ and CaCO3. Also, it has a positive effect on chlorophyll, aerial, root biomass and on lettuce growth in general. The results also have indicated that the 500g dose of earthworm casts/pot have been increased the various parameters.
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    Effect of storage time on the biodegradability of olive oil mill wastewater from the cold extraction of olive oil system
    (The EuroBiotech Journal, 2021) Kenza Kadi; Dalila Addad
    The olive oil processing industry’s liquid effluents (OMW) have a polluting capacity for the ecosystems. This study aimed to evaluate the physicochemical quality of OMW of two varieties of separated and combined olives cultivated in Khenchela Eastern Algeria, from the cold extraction of the olive oil extraction system. These was to determine their degree of pollution and biodegradability during one year of storage at ambient temperature to recommend the correct treatment for each storage time. Results of the measured parameters pH, EC,TSS percent, H 2 O percent, lipids, DM, OM, MM, VM, COT percent, NTK percent, C/N, BOD 5 , COD, BI, TOM, BOD 5 /COD show that wastewater from olive oil mills has an acid pH, and they are very loaded with organic matter evaluated in terms of COD and BOD 5 , quite filled with minerals. The storage of olive mill waste can reduce progressively the pollution caused by this waste. Whereas during one year, the reduction rate of COD, BOD 5 , TOM, BI, is respectively 29.4%, 54.8%, 39.16%, 54.2%, but C/N, BOD 5 /COD continue to increase as well as pH that continues to decrease during the storage. Accordingly, storing olive mill waste during a year reduces its pollution rate, so it is slowly biodegradable. When disposing of it, an adequate treatment procedure must be required to protect the environment.
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    HPAEC-PAD, biochemical characterization, and evaluation of the antioxidants activities of polysaccharides extracted from Olive Mill Wastewater of two endemic varieties of Khenchela region, Algeria
    (journal homepage, 2023) Kenza Kadi; Dalila Addad
    Olive Mill Wastewater (OMWW) is considered to be one of the by-products of the extraction of olive oil that causes serious problems to the environment. This study describes a qualitative and quantitative analysis of biochemical characterizations and assessment of polysaccharides antioxidant activities from cold extraction of two endemic varieties from Khenchela region, eastern Algeria. As a result, the physicochemical characteristics of these OMWWs (Chemlal and Ferkani) were found to be acidic (pH: 4.77–5.1) and saline (1.35–1.40 mg/l). According to Chemlal and Ferkani, the dry matter (DM), total suspended solids (TSS) and mineral matter (MM) are respectively (159.3 ± 14.03 g/L, 4.13 ± 0.04 g/L, 10 ± 0.5 g/L and (117.4 ± 12.03 g/L, 1.06 ± 0.1 g/L, 9.7 ± 1 g/L). In addition, the COD and DBO5 requirements are (9625.6 ± 13.42 mg/l; 45 ± 08 mg/l for Chemlal) and (10490.6 ± 23.84 mg/l; 440 ± 3 mg/l for Ferkani) respectively. Due to their high content of organic matter and mineral salts, the OMWW studied have very little potential for biodegradation. Three extracts were prepared for each cultivar: insoluble alcohol fraction (AIR), soluble water fraction (SF) and insoluble water fraction (IF). The biochemical characterisation was carried out by colorimetric assays using an appropriate spectrophotometer for the determination of total sugars (OT), reducing sugars (OR), neutral sugars, protein, nitrogen and phenolic compounds. The qualitative and quantitative analysis of the polysaccharides was carried out using high- performance anion-exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD). The results obtained showed heterogeneous composition of nine monosaccharides for each fraction at different levels. The total dry matter (%DM) of the Chemlal fractions (AIRC, SFC, IFC) are respectively (14.44%, 16.93%, 15.68%) and those of the Ferkani fractions (AIRF, SFF, IFF) are respectively (10%, 15.05%, 10.52%). The antioxidant activities of two soluble fractions (SFC and SFF) were evaluated using five assays: DPPH, ABTS, CUPRAC, phenthroline and hydrogen peroxide, when the best antioxidant activity is provided by hydrogen peroxide.
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    IMPACT OF EARTHWORM ACTIVITY ON THE CHEMICAL FERTILITY OF IRRIGATED SOIL WITH URBAN EFFLUENTS
    (Journal of Fundamental and Applied Sciences, 2017-09-01) Dalila Addad; Nawel Ababsa
    The reuse of urban effluents to irrigate the soils of peri-urban grasslands in the vicinity of the town of Setif (northeastern Algeria) is an old and widespread practice. In this context, the present study was conducted to evaluate the effect of the irrigation with urban effluents on the biological and chemical behavior of soils. Effluents analysis showed significant organic and particulate pollution, the latter contributed to earthworm abundance and increased the richness of irrigated soils with nutrients. The analysis of turricules revealed the role of earthworms through the activity of bioturbation in the increase of the rate of organic matter as well as in the bioavailability of the nutrients of the irrigated soils. In space, permanent vegetation cover has played an important role as a biofilter. This was confirmed by the inter-site differences recorded through the measured variables particularly organic ones.
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    Long-term effects of wastewater reuse on hydro physicals characteristics of grassland grown soil in semi-arid Algeria
    (Journal of King Saud University – Science, 2019-10-08) Nawal Ababsa; Dalila Addad
    abstract The raw and treated wastewaters are often evacuated downstream of settlements and widely reused in pre-urban agricultural irrigation. Our study highlights the impact of wastewaters on the soil hydrophysical properties as well as biological activity. Our study was conducted in eastern part of Algeria, on long-term (>60 years) wastewater irrigated grassland to determine the biological component and hydrodynamic soil behavior under these practices. Effects of three wastewater types (raw urban, treated and agricultural effluents) on soil were studied and water was characterized both physically and chemically. Assessment of the effects involved soil porosity, soil hydraulic conductivity and earthworms’ abundance. The results revealed that waters contain high concentrations of organics (BOD 5&COD) and suspended solids (SS). Hydro-physical properties and biological activity showed that irrigation with raw urban wastewater enhances soil earthworm density, porosity and higher water transfer via hydraulic conductivity. Biological activity resulted in ideal pore architecture for materials and solutes transfer, induced a variety of micro morphological transformations in relation to the abundance of earthworm communities mostly endogeic and anecic.
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    Maceration and Liquid-liquid extractions of phenolic compounds and antioxidants from Algerian olive oil mill wastewater
    (Research Article, 2022-04-13) Kenza Kadi; Dalila Addad
    Olive oil mill wastewater (OMW) is a major waste stream from the olive oil industry. It is highly polluted due to phenolic compounds. The present study focused on the physicochemical properties of OMW as well as the quantitative and qualitative effects of two methods of extraction (maceration and liquid-liquid extraction) of phenolic compounds.
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    Phenolic compounds and biological activities of phenolic extract of olive oil mill wastewater issue from the cold extraction of olive oil from Khenchela (Algeria)
    (Research Article, 2021-04-12) Kenza Kadi; Dalila Addad
    This study aimed to the determination of antioxidant, anti-inammatory, and anticoagulant activities of phenolic compounds present in olive oil mill wastewater (OMW) issue from the cold extraction of olive oil from Khenchela eastern of Algeria.After polyphenols extraction, a quantity and quality analysis by LC-MS was made. The LC-MS (liquid chromatography–mass spectrometry)results were revealed the presence of 20 phenolic compounds in theextract of OMW which were: (quinic acid, gallic acid, protocatechuic acid, caffeic acid, p-coumaric acid, rutin, transfrolic acid, hyperoside (quercetin-3-o-galactoside), luteolin-7-oglucoside, naringin, 4.5-di-caffeoyquinic acid, quercetrin(quercetin-3-o-rhamonosid), apegenin-7-oglucoside, salviolinic acid, kampherol, quercetin, naringenin, apegenin,cirsiliol,andcirsilineol).TheresultsofantioxidantactivitywithDPPH(2,2-diphenyl-1-picrylhydrazyl),ABTS+(2,2'-azino-bis(3ethylbenzothiazoline-6-sulfonicacid),andFRAP(ferricreducingabilityofplasma)showed that theIC(half-maximal inhibitory concentration) were(9.62 ± 0.28µg/mL, 7.10 ± 0.11µg/mL and 3.59 ± 0.24µg/mL)respectively. The extract of OMW was found to exhibit the highestinhibitory effectanti- 50 inammatory activity usinginhibition of protein denaturation(IPD) and membrane stabilizing potential (MSP) tests (80.46 ± 3.81; 87.43 ± 0.66 µg/mL) more than the standard used. In addition, the extract had the best anticoagulation activity in the endogenous and exogenous pathways (44.77 ± 0.25s; 15.84 ±  0.12s). Based on these results, it is right to conclude that OMW is an important source of natural phenolic compounds that have important antioxidant, anti-inammatory, and anticoagulant activities.
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    Phytochemical Screening and Radical Scavenging Activity of Whole Seed of Durum Wheat (Triticum durum Desf.) and Barley (Hordeum vulgare L.) Varieties
    (Jordan Journal of Biological Sciences, 2017-09-04) Sofia Hamli; Kenza Kadi; Dalila Addad
    Three durum wheat (Triticum turgidum L. var. durum) cultivars, namely Bousselam, Vitron, and Gaviota durum, and one barley genotype (Hordum vulgare L.), Fouara, grown under semi-arid conditions were compared for their total phenolic and flavonoids content and antioxidant activities. Antioxidant activity was tested using DPPH radical scavenging assay method. The phytochemical screening revealed the presence of tannins, flavonoids, coumarins, saponins and phenolic compounds in each variety seeds. The results of the present study indicate significant differences among the evaluated varieties in terms of total phenolic and flavonoid contents and for radical scavenging capacity. Among the tested varieties Gaviota durum showed high total phenolic (95.32± 0.27 μg /mg) and flavonoid content (78.80 ± 0.27 μg/mg) and an intermediate radical scavenging capacity. While barley variety Fouara expressed high radical scavenging capacity (54.8% ±0.34) and intermediate total phenol and flavonoids contents. The tested durum wheat and barley varieties possessed varying but meaningful antioxidant activities which were not significantly correlated to their phenol and flavonoid contents. It is necessary to ensure that increased bioactive components in grains are combined with good agronomic performance, high grain yield and high quality for processing. The results of the present study should have significant implications for plant breeders as well as for grain and food processors.