Browsing by Author "Aiche Mohamed Amine"

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    Biochemical and Hematological Changes Following Long Term Exposure to Mancozeb
    (Advances in Bioresearch, 2015-01-09) Aiche Mohamed Amine
    The aim of this study is to evaluate the effects of mancozeb; a carbamate fungicide on some hematological parameters and some biochemical indices related to kidney and liver function. Male wistar rats were given different doses of mancozeb orally: 500 and1000 mg/body weight/day for 8 weeks. The obtained results showed an increase in the absolute weight of liver, spleen and kidneys, mainly in the group exposed to 1000 mg/ kg. Hematologic study revealed a decrease in red blood cells hemoglobin and hematocrit values in all treated groups. However, white blood cells witnessed an increase in all the treated groups. The biochemical tests revealed a rise in liver transaminases (AST / ALT), alkaline phosphatase and total bilirubin serum, while total protein levels and total lipids have significantly decreased mostly in group treated with 1000 mg/kg. Urea and creatinine have significantly increased in the group treated with 1000 mg/kg. In the light of these results, it appears that chronic mancozeb exposure produced biochemical and hematological changes of some parameters related to the liver and kidneys function
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    SUBCHRONIC MANCOZEB TREATEMENT INDUCED LIVER TOXICITY VIA OXIDATIVE STRESS IN MALE WISTAR RATS
    (Comm. Appl. Biol. Sci, Ghent University, 2014) Aiche Mohamed Amine
    Mancozeb is a manganese/zinc ethyleneͲbisͲdithiocarbamate fungicide that is widely used in agriculture to control a broad variety of fungal infections of both vegetables and ornamental plants. The present study has been carried out to investigatethe possible effect of mancozeb on animal the oxidative stress and some of the biochemical markers in male wistar rats. In this experiment, adult male rats weighing between 200 and 250 g were treated per osfor 4 weeks with two different doses of 800 and 1200 mg/kg per day. Reduced glutathione (GSH) levels were decreased in all treated groups compared to control ones. It has been observed a significant increase in the fresh weight of liver in individuals of both doses. MoreͲ over, mancozeb exposure caused a signiÞcant (pޒ0.05 (fall in aspartic aminotransferase (AST) and alanine aminotransferase (ALT) in group treated with 1200 and 800 mg/kg/day. Similarly, alkaline phosphatase (ALP) activity underwent a signiÞcant (pޒ0.05 (increase in both groups. The obtained observations clearly reveal hepatotoxic effects of mancozeb in rats and constitute, therefore, an environmental health risks to living organisms.
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    Thyroid Disruption and Infertility after Chronic Exposure to Mancozeb
    (Advances in Environmental Biology, 2015-04-19) Aiche Mohamed Amine
    This work aims to highlight the toxic effects of mancozeb; polymeric complex of manganese and zinc ethylene bis-dithiocarbamate, on the fertility and thyroid functions. Male Wistar rats were treated orally with different doses of mancozeb: 250, 500 and 1000 mg/kg body weigh/ day for 8 weeks. The obtained results showed a highly significant increase (p≤ 0.01) on thyroid absolute weight in the groups treated with 500 and 1000 mg/kg body weight/day respectively. However, testes absolute weight witnessed a significant decrease (p≤ 0.05) in group treated with 500 mg/kg, this decrease becomes very highly significant in the group treated with 1000 mg/kg/day. The biochemical test revealed a significant increase of cholesterol and triglycerides rates in the group treated with the highest dose. A highly significant increase of blood glucose is also observed in the groups treated with 500 and 1000 mg /kg/day. Hormone assays have shown a very highly significant decrease of thyroxine in all the groups treated with different doses, while triiodothyronine concentration witnessed a very highly significant decrease only in the group treated with 1000 mg/kg/day. Finally, the studying of biological characters of the spermatozoa showed a highly significant and very highly significant decrease in the concentration of spermatozoa and their mobility in rats treated with 500 and 1000 mg/kg/day respectively. In conclusion, it seems that long term exposure to mancozeb is associated to hypothyroidism by decreasing FT4 and FT3 and has adverse effects on testes function leading to physiological impairment.
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    Toxicity of Subchronic Doses of Propiconazole, Propineb and Their Mixture on Reproductive Parameters in Male Rats
    (Advances in Environmental Biology, 2015-01-28) Aiche Mohamed Amine
    The present study has been carried out to elucidate the toxic effects of two commonly used pesticides, propiconazole, propineb and their mixture on reproductive parameters in male wistar rats. Twenty eight male rats were randomly divided into four groups. Rats in groups 2, 3, and 4 received propiconazole 60 mg/Kg, Propineb 100 mg/Kg and their mixture 30 mg/kg Propiconazole + 50 mg/kg Propineb, respectively, while those of group one served as control , and that throughout the 4 weeks experimental period. Animals were observed for clinical toxicity. At the end of treatment, all groups of animals were scarified and samples of different organs were fixed in the formol 10% for histopathological study. The semen study showed a decrease in the count and mobility of spermatozoa, accompanied with a significant decrease in morphological changes of flagellum in the treated groups especially with mixture compared to control group. It was also a decrease in the weight of the testis and epididymis in the treated groups compared to the control. Histological changes were observed in the testis and epididymis in all treated groups. In conclusion this paper shows clearly that the treatment with these pesticides alone or in combination for 4 weeks can induce a decrease in the quality of the sperm with histopathological changes in the testes and epididymis.