Browsing by Author "Abed Mohamed Afoune"

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    A novel and ultrasensitive non‑enzymatic electrochemical glucose sensor in real human blood samples based on facile one‑step electrochemical synthesis of nickel hydroxides nanoparticles onto a three‑dimensional Inconel 625 foam
    (Journal of Applied Electrochemistry, 2023) Rafiaa Kihal; Hassina Fisli; Mohamed Lyamine Chelaghmia; Widad Drissi; Chahira Boukharouba; Sara Abdi; Mouna Nacef; Abed Mohamed Afoune; Maxime Pontié
    In this contribution, a simple and novel non-enzymatic electrochemical sensor for the detection of glucose was successfully prepared by direct in situ growth of nickel hydroxide nanoparticles (Ni(OH)2NPs) onto a three-dimensional Inconel 625foam (IN625F) substrate through a facile electrochemical route, using cyclic voltammetry (CV) method in alkaline medium without addition of nickel salts. Then, surface characterization of modifed Ni(OH)2/IN625F electrodes was carried out through advanced technologies, such as scanning electron microscopy (SEM) and X-ray difraction (XRD). The electrochemical catalytic behavior of the fabricated electrodes was investigated using CV and amperometric methods. The results revealed that the novel modifed sensor, Ni(OH)2/IN625F, showed the highest sensitivity of 5685 μAmM−1 cm−2 over a wide linear concentration range from 1 to10 mM, with lowest detection limit (LOD) of 2 μM (S/N = 3), and short response time within<2 s. Therefore, the proposed non-enzymatic electrochemical sensor demonstrated high selectivity and stability, good reproducibility, and low cost. In addition, analysis of human blood samples was performed. Hence, the constructed glucose sensor, Ni(OH)2/IN625F, with suitable performance could be used as a promising material in real human blood samples.
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    Dendritic Cu(OH)2 nanostructures decorated pencil graphite electrode as a highly sensitive and selective impedimetric non‑enzymatic glucose sensor in real human serum blood samples
    (Monatshefte für Chemie - Chemical Monthly, 2022) Chahira Boukharouba; Mouna Nacef; Mohamed Lyamine Chelaghmia; Rafiaa Kihal; Widad Drissi; Hassina Fisli; Abed Mohamed Afoune; Maxime Pontié
    In this study, an efective and low price non-enzymatic electrochemical glucose sensor was easily elaborated through electrodeposition of highly uniform copper dendrites hydroxide onto pencil graphite electrode (Cu(OH)2/PGE). The obtained electrode was investigated by feld-emission scanning electron microscopy, atomic force microscopy, energy-dispersive X-ray spectroscopy, X-ray difraction, and FT-IR characterizations. The electrocatalytic properties of the modifed electrode were investigated by cyclic voltammetry, amperometry, and electrochemical impedance spectroscopy techniques, which can be readily applied to determine glucose using the fabricated sensor, as the results after optimization revealed. Furthermore, a single frequency impedance method was applied for glucose determination as an alternative to conventional EIS methods. The fabricated Cu(OH)2/PGE electrode exhibited a selective impedimetric response towards glucose over an exceptional linear range from 0.1 to 12 mM (R2 = 0.999) with a detection limit of 71.8 µM. Finally, Cu(OH)2/PGE was successfully applied to the assay of glucose in blood samples with unknown interferences.