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Browsing scientific events by Author "Oumaima NAILI"

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    Phosphate removing using bacteria isolated from activated sludge for wastewater remediation
    (Journal of Biodiversity and Environmental Sciences, 2015) Oumaima NAILI
    Abstract The objective of the current study is to isolate phosphate removing microbes from activated sludge for wastewater remediation in the area of Khenchela (Eastern Algeria) . Phosphate rate was determined using colorimetric method and batch tests were developed to evaluate the biomass composition of the sludge. Four efficient pure strains isolated from activated sludge samples and identified as Acinetobacter junii, Pseudomonas aeruginosa, Moraxella lacunata, Alcaligenes denitrificans were tested for their capacities of phosphate reduction. The best rate of phosphate removal by pure cultures is observed with Acinetobacterjunii, 76%, followed by Alcaligenes denitrificans, 70.42%, Pseudomonas aeruginosa, 61.78%, and Moraxella lacunata, 50.6%. Our results show that applying of mixed bacterial culture containing mostly isolated strains for bioremediation purpose can be used successfully for the elimination of phosphate from activated sludge of wastewater treatment plants.
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    Probiotic potential of lactobacillus strains isolated from fresh bee pollen
    (Scholars Research Library, 2016) Oumaima NAILI
    ABSTRACT The present work aimed to evaluate the probiotic potential of lactobacillus strains isolated from fresh bee pollen. Thirty three lactobacillus isolates were screened for antagonistic activity against six pathogenic bacteria. Ten of them were selected identified as lactobacillus plantarum and evaluated for resistance to acidic pH (2 and 3) and 0.3% bile salts, hydrophobicity and autoaggregation ability. Moreover, their safety was verified by testing haemolytic activity on human blood agar and antibiotic resistance. The results showed that all lactobacillus strains were effective against all indicator bacteria; all strains were able to maintain their viability after 3h exposure to pH 3 and 4h in the presence 0.3% of bile salts. While only five could survive with losses in cell viability after 3h exposure to pH 2. Most strains showed a high hydrophobicity and autoaggregation ability. All lactobacillus strains were resistant to ciprofloxacin, tobramycin, nalidixic acid and colistin. 50% of the strains were susceptible to chloramphenicol, Nitroxolin, penicillin G, Cefoxitin, pristinomycin, cefexim and 80% are susceptible tostreptomycin. No haemolysis was observed on blood agar. Five strains of Lactobacillus plantarum were selected as suitable candidates for industrial use.