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Browsing scientific publication and books by Author "BENDJEMANA KATIA"

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    CYP1A1 GENE POLYMORPHISM AND NASOPHARYNGEAL CARCINOMA IN ALGERIAN POPULATION
    (Current Trends in Natural Sciences, 2018) BENDJEMANA KATIA
    Abstract CYP1A1 polymorphisms are known to play a crucial role in the development and metastasis of malignant diseases including nasopharyngeal cancer (NPC). Individual susceptibility to cancer from environmental agents may be influenced by polymorphic metabolic gene (CYP1A1). The present study was carried out to analyze the relationship between CYP1A1 genetic polymorphism and the risk of developing nasopharyngeal cancer (NPC). The study involved 70 patients with nasopharyngeal carcinoma, compared with 100 healthy controls. Genomic DNA samples were assayed for restriction fragment length polymorphisms in the CYP1A1 by PCR amplification followed by digestion with MspI and BsrDI. The frequency comparison between the two groups was determined by the χ(2) test. Showed that the distribution of CYP1A1 mt/mt allele frequency was found to be significantly different between controls (6%) and cancer cases (11%) along with twice increase of the NPC risk in Algeria (OR=2.5, CI 95% [1.72-2.30]) Our results are in line with those found for other populations and subsequent they emphasize the importance of the involvement of this gene in the development of the NPC detoxification process, which exhibited more and more strong association with environmental factors.
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    EFFECT OF PGPR INOCULATION ON DURUM WHEAT GROWTH VARIETIES
    (Global Science Publications, 2020-09-15) BENDJEMANA KATIA
    Abstract – The purpose of this work is to isolate PGP-effect rhizobacteriaand to evaluate their potential for durum wheat varieties. The isolation of the bacteria was done using the suspension-dilution process. Eleven bacterial isolates were isolated from the rhizosphere of hard wheat. The latter were described by molecular characterization, based on the sequencing of RNA 16s. Once established, these isolates were characterized by their traits of growth promotion, such as biological control through the testing of the anti-fungal activity which was performed to two species of Fusarium. The ability of stimulating growth of durum wheat varieties was assessed in vivo. The molecular identification of eleven isolates showed 98-99 % of our isolates to species of the following genera: Enterobacter, Enterobacter asburiae, Paenibacillus glucanolyticus and Serratia sp. Research on the plant growth promoting traits in our isolates, showed that 80% of isolates have shown positive results with qualitative and quantitative estimates of siderophores, as well as for solubilized phosphate. All of our isolates showed a similar result with production of ammonium, cellulase, pectinase and eight isolates released AIA at different concentrations; whereas only three isolates could release HCN, and four isolates did not produce the protease. Similarly, direct inhibition of the growth of Fusariumsp species was observed, with significant improvement in the different growth parameters of the durum wheat varieties being studied. This research concluded that the findings obtained so far demonstrated that our isolates have phytostimulative, and phytoprotective behaviors. The latter opens up the possibility of their use in future laboratory studies in order to generate biofertilizer inoculants.
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    Effect of using treated wastewater on the bacteriological quality of raw cow's milk: A case of a farm in Northeastern Algeria
    (African Journal of Microbiology Research, 2020-04-27) BENDJEMANA KATIA
    This study aims to assess the impact of the use of treated wastewater (without chlorination) in farming and dairy cattle breeding. Milk samples were collected from a farm in northeastern Algeria. The treated wastewater from the treatment plant is used on this farm for different activities. The results obtained show that the average contamination of milks with total flora is 3.7.105 CFU /ml. Fecal coliforms are present at an average value of 1.5.103 CFU/ml. All of the samples (100%) were positive for the count of fecal enterococci with an average value of 2.5.10 CFU/ml. Fungal flora was present with an average value of 1.36,103 CFU/ml. Escherichia coli was isolated in 100% of the samples with high resistance rates for beta-lactam antibiotics. The results obtained for the search for pathogens belonging to the genus Staphylococcus show that 64% of the isolates were coagulase-negative Staphylococcus and 36% of the isolates were coagulase-positive. The study of Staphylococcus susceptibility/resistance to antibiotics revealed high frequencies of resistance, especially to beta-lactam antibiotics and macrolides. The bacteria tested show a majority resistance for Penicillin and Oxacillin (100%). These results reflect the microbiological risk that the consumption and marketing of this milk represents for the health of consumers and the need to implement preventive measures.
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    Étude du profil épidémiologique et des facteurs de risque alimentaires du cancer du nasopharynx dans le Nord-Est algérien
    (springer, 2010-12-06) BENDJEMANA KATIA
    Nasopharyngeal carcinoma (NPC) is one of the most important cancers in Algeria with 8 to 12 cases/ 100,000 persons a year. The pathogenesis of NPC is not clear. In Algeria and Tunisia, it represents the first most common cancer of the larynx in women and the second most common tumor inmen. This is a retrospective epidemiological study of the NPC in the North-East of Algeria and evaluates the impact of the consumption of some foods, tobacco and alcohol on the risk of development of NPC. The study of a group of 160 patients affected by NPC, using 205 people as a control group, has shown that this cancer was predominant in men, particularly in the population between 40- and 59-years-old. The analysis has shown that preservatives such askhliiseem to be a statistically significant factor related to NPC. Our results also show a strong association between alcohol consumption and tobacco use and the risk of developing NPC. However, we did not find a statistically significant relationship with other food factors. Résumé Le carcinome du nasopharynx (CNP) représente un des cancers les plus importants dans notre pays, avec une incidence moyenne de 8 à 12 cas/100 000 habitants par an. Il représente en Algérie et en Tunisie le premier cancer des voies aérodigestives chez la femme et le deuxième après le cancer du larynx chez l’homme. L’étiologie de ce cancer incrimine trois types de facteurs: le facteur viral, les facteurs génétiques et environnementaux, notamment alimentaires et tabagiques. Le but de notre travail rétrospectif est d’évaluer le profil épidémiologique du cancer du nasopharynx au niveau des régions de Khenchela et de Batna et leurs environs et d’identifier les aliments à risque pour le CNP. L’étude a concerné 160 patients ayant un CNP de type UCNT (undifferentiated carcinoma of nasopharyngeal type) recensés dans les régions et 205 témoins appariés aux malades selon l’âge, le sexe et la région géographique. Les résultats montrent que ce cancer prédominait particulièrement chez les hommes, avec une forte incidence pour la tranche d’âge de 40 à 60 ans. L’analyse des facteurs alimentaires et tabagiques montre un risque élevé de CNP lié significativement à la consommation de produits de conservation traditionnelle tels que le khlii et les saumures. Nos résultats montrent aussi une forte association entre la consommation d’alcool et de tabac et le risque de développement du CNP. Cependant, cette association n’a pas pu être démontrée pour le reste des aliments faisant l’objet de notre étude.
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    GSTM1 and GSTT1 polymorphisms, tobacco use as risk factors for nasopharyngeal carcinoma in maghreb population — A case-control study
    (Springer, 2013-09-09) BENDJEMANA KATIA
    Abstract Nasopharyngeal carcinoma (NPC) has known environmental risk factors, notably smoking, and enzymes that biotransform carcinogens. The interindividual differences observed in the metabolism of tobacco carcinogens substances have been attributed to the genetic polymorphism of genes, which code for enzymes involved in detoxification. Polymorphic deletions of GSTM1 and GSTT1 genes involved in the detoxification of potentially carcinogenic agents may be a risk factor for NPC. To investigate the roles of genetic variations of GSTM1 and GSTT1 in NPC susceptibility in the Northern African population, we conducted a case–control study of 132 NPC cases and 200 controls. DNA was isolated from blood for cases and controls. GSTM1 and GSTT1 deletion variants were genotyped by multiplex PCR assays. Allelic frequencies between the two groups were compared using a χ2 test, and odds ratio (OR) with 95% confidence intervals were calculated. The results indicate that an excess of the GSTM1 null genotype was observed in cases compared with controls, 50% versus 33%. The genotypic frequency of GSTM1 null genotype between controls and patients was significantly different. This genotype confers an increased risk of NPC [OR = 2.18; CI 95% (1.23–5.33). The association between GSTM1 and increased NPC risk in this cases-controls study was present among both men [OR = 2.59 ; CI95% (1.81–4.32)] and smokers. No significant association was observed between null GSTT1 genotype and the risk of CNP.
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    Morphological, molecular, and biochemical study of cyanobacteria from a eutrophic Algerian reservoir (Chefa)
    (Environmental Science and Pollution Research, 2022-01-04) BENDJEMANA KATIA
    The cyanobacteria management in water bodies requires a deep knowledge of the community composition. Considering the reliable and thorough information provided by the polyphasic approach in cyanobacteria taxonomy, here we assess the cyanobacterial community structure of the Chefa reservoir from Algeria. Cyanobacteria were identifed on the basis of morphological traits and next-generation sequencing (NGS); toxins-related genes were localized in addition to the identifcation of toxins; temperature and nutrient level of water samples were also determined. The polyphasic approach was essential for cyanobacteria investigation; 28 genera were identifed through 16S rRNA metabarcoding with the dominance of taxa from Microcystis (34.2%), Aphanizomenon (20.1%), and Planktothrix (20.0%), and morphological analysis revealed the association in this water body of fve species within the genus Microcystis: M. aeruginosa, M. novacekii, M. panniformis, M. ichthyoblabe, and M. fos-aquae. The presence of mcyE genotypes was detected; moreover, HPLC–PDA and LC–ESI–MS/ MS revealed the production of microcystin-LR. Results obtained in our study are very important since this ecosystem is used for water supply and irrigation; as a consequence, a good water management plan is essential.
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    Polymorphisme génétique des enzymes gluthation-S transférases et N-acétyl-transférases 2 dans le cancer du nasopharynx en Tunisie
    (nasopharynx en Tunisie, 2016) BENDJEMANA KATIA
    Les différences interindividuelles observées dans le métabolisme des xénobiotiques peuvent être dues aux polymorphismes des gènes codant pour les enzymes de détoxication. Cette variabilité génétique semble être associée à des différences de susceptibilité à certains types de cancers, dont le carcinome du nasopharynx. Notre travail vise à établir les fréquences génotypiques des polymorphismes d’ADN dans les gènes de trois enzymes de détoxication : les gluthation-S-transférases (GSTM1 et GSTT1) et la N-acétyl-transférase 2 (NAT2). L’étude a porté sur 45 malades présentant un carcinome du nasopharynx, comparés à 100 témoins sains. La présence de la délétion homozygote des gènes GSTM1 et GSTT1 est déterminée par PCR multiplex. L’étude du polymorphisme nucléotidique du gène NAT2 utilise l’approche PCR-RFLP. La comparaison des fréquences entre les deux groupes est établie par un test χ 2 et le calcul des odds ratio avec un intervalle de confiance à 95 %. Nos résultats montrent une différence significative entre les deux groupes concernant le génotype GSTM1 nul. Celui-ci est associé à une augmentation du risque de carcinome du nasopharynx (odds ratio = 2,12, IC95 % = 1,03-4,37). Par ailleurs, la fréquence du génotype NAT2*6/NAT2*6 est plus élevée chez les patients. Le calcul de l’odds ratio montre une association entre ce génotype et le carcinome du nasopharynx. En conclusion, l’augmentation du risque de développement du carcinome du nasopharynx en Tunisie semble être associée aux génotypes GSTM10/0 et NAT2*6/6.
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    RECHERCHEDosage des marqueurs tumoraux CYFRA 21-1 et ACEdans le cancer du nasopharynx en Algérie
    (elsevier, 2013-03-09) BENDJEMANA KATIA
    Nasopharyngeal cancer is one of the most important cancers in our country. It ischaracterized by the influence of different etiologic factors: viral, genetic and environmental.Its diagnosis is delayed by lack of specific tumor markers. The aim of this study was to evaluatethe importance of the use of CYFRA 21-1 and ACE markers in nasopharyngeal carcinoma (NPC).Our prospective study interested 164 patients (132 M, 32 F) with a mean age of 47 years (13 to 70)with 24 of them aged less than 30 years, presenting a nasopharyngeal carcinoma histologicallyconfirmed and 180 healthy controls without evident neoplasm. Undifferentiated forms represent93% of cases. A blood sample was collected from each patient and control before any treatment,as well as controls to measure CYFRA 21-1 and ACE by immunoenzymatic assay. The resultsshowed that the mean serum CYFRA 21-1 values were significantly higher in patients with NPCthan those in controls (P ± 0.001). A significant correlation was found only between the serumCYFRA 21-1 level before treatment and the clinical outcome of patients (P ± 0.0009). Seric levelof CYFRA 21-1 at diagnosis of NPC may play a predictive role to evaluate the risk of metastaticdisease and prognosis.