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Amylolytic and antibacterial activity of filamentous fungi isolated from the rhizosphere of different plants grown in the Tamanghasset region
(Heliyon, 2023-03-09) Anis BERTELLA
In the present study, we were interested in studying the amylolytic and antibacterial activity of some filamentous fungi isolated from the rhizosphere of cultivated plants in Tamanghasset region. Consequently, 11 pure strains belonging to the different fungal genera were isolated Alternaria, Aspergillus, Cladosporium, Curvularia, Fusarium, Mucor and Penicillium. Positive result of amylolytic activity was revealed on all the isolated strains, with important hydrolysis zones of 54.33 ± 1.15 mm, 54.00 ± 3.61 mm, 52.00 ± 6.08 mm and 51.33 ± 15.01 mm for Aspergillus sp.1, Curvularia sp., Fusarium sp.2 and Mucor sp. respectively. In addition, analysis of variance (ANOVA) of the means of hydrolysis zones diameters shows that the values linked by the same letter do not show any significant difference at P < 0.05. Antibacterial activity of the isolated fungal was demonstrated by the agar cylinder technique against four pathogenic bacterial strains. The results showed a variability of the inhibition zones, thus the most important results were recorded against S. aureus, E. coli and K. pneumonia for all fungi which produced inhibition zones ranging from 15.33 ± 0.00 to 23.66 ± 1.71 mm. while all isolate had the lowest inhibition zone against P. aeruginosa. In conclusion, the obtained results indicated the isolated filamentous fungi have the potential to inhibit the four pathogenic bacterial strains, S. aureus, E. coli, K. pneumonia and P. aeruginosa, while simultaneously showed significant amylolytic activity.
Artemisia herba-alba Asso. essential oil antibacterial activity and acute toxicity
(elsevier, 2018-02-27) Anis BERTELLA
Artemisia herba-alba Asso., known as the desert wormwood, is a medicinal plant and its essential oil is used in Algerian herbal medicine. In the present study the in vitro antibacterial activity against 21 bacterial strains and chemical composition of Artemisia herba-alba essential oil were investigated. The acute toxicity by determination of the median lethal dose was also studied. The results of gas chromatography/mass spectrometry analysis of the essential oil gave 19 compounds accounting for 98.7% and the major constituent was camphor with an amount of 50.7%. A significant antibacterial effect was observed with important zones of inhibition against Klebsiella oxytoca (31.3 mm) by disc diffusion method and against Acinetobacter baumannnii (47.6 mm) by microatmoshphere method. The minimum inhibitory concentration and the minimum bactericidal concentration values were ranging from 5 to 10 mg mL−1 and 10–20 mg mL−1 , respectively. Moreover, their ratio exhibited by the essential oil was 2. The bactericidal end point was achieved after 24 h of exposure to the essential oil, for all the bacteria assayed. The oil was slightly toxic with a median lethal dose of 615 mg kg−1 . The results of this study suggest that the essential oil of Artemisia herba-alba can be a source of natural antibacterial agents with potential pharmacological applications.
Chemical constituents of the aerial parts of Algerian Galium brunneum: Isolation of new hydroperoxy sterol glucosyl derivatives
(elsevier, 2020-05-11) Anis BERTELLA
The liposoluble extract of Galium brunneum aerial parts from North-eastern Algeria was chemically investigated. The EtOAc soluble portion contained a series of glycosyl cucurbitacins and sterols including three new glucosyl hydroperoxy sterols 1–3 among other phenolic components whereas the BuOH soluble fraction was dominated by glycosyl derivatives of flavonoids, iridoids and lignans, according to the chemistry reported in the literature for the genus Galium. The structure of new oxidized sterols 1–3 was determined by spectroscopic methods as well as by comparison with related known metabolites. Selected main compounds from both extracts, which revealed moderate antibacterial activities, were tested for their growth inhibitory properties against Gram-positive and Gram-negative bacteria. This is the first report of cucurbitacins in plants of genus Galium.
Consequences of Lack of Hygiene in Fast Food in The City of Oran
(Journal of Purity, Utility Reaction and Environment, 2016-10-01) Anis BERTELLA
ABSTRACT In fast food, the most important factor is HYGIENE where several measures and precautions must be taken to avoid contamination of food for consumption. If the elementary rules of hygiene are neglected; it follows that the meals are considerable risks due t harmful microorganisms to the health microbiological quality meals at fast food in four sites in Oran: B), a popular destricts restoring in the Centre town (site C) and finally a university canteen in Senia (site D). Samples of food (cooked and served meals), surfaces, equipment and hands were made. Then, the of some physicochemical parameters was deteriorate the quality of food (mesophile aerobic flora, total and faecal coliforms, sulphite anaerobes, Staphylococcus aureus The results obtained in the site (Site D) in the four studies showed good microbiological quality of foods unlike other ill-prepared and stored or located in areas not respecting the cold chain. The results got for the university canteen ( with the difference in the 3 other sites What gives the proof of a bad compliance with the rules of hygiene during storage? However, some recommendation by ensuring a rigorous and strict bacteriological monitoring to avoid possible collective food illness due to bacterial contamination of food offered to consumers and by ensuring a work quality.
Correlation between Hydrolytic Enzymes Activity, Geographical Origin and Pathogenicity of Some Isolates of Fusarium oxysporum f. sp. albedinis
(Archives of Pharmacy & Pharmacology Research, 2018-10-05) Anis BERTELLA
The 20 isolates of Fusarium oxysporum f. sp. albedinis (Foa) produce amylase and cellulase enzymes, with the development of lysis zones around the inoculum (clear region) on the selective medium, indicates starch hydrolysis and cellulose degradation, with a variation in the ability of the isolates to produce these two enzymes, and this after measuring the diameters of the lysis zone. After Principal Component Analysis (PCA), we found that there is a strong positive correlation between mortality rate and amylolytic activity CP1 (74.80%) on the one hand and a weak correlation with cellulase CP2 (22.3%), on the other hand. While, there is no correlation between the pathogenicity of the isolates and the geographical origin of the isolate or cultivar from which this isolate was isolated P=0.3042.
FRYING OIL QUALITY ASSESSMENT IN BATNA CITY (ALGERIA) AT FAST FOOD RESTAURANTS
(Annals. Food Science and Technology, 2018-09-28) Anis BERTELLA
The oil quality used in deep frying can affect both, the fried food properties and the consumer’s health. In the present work, the frying oils quality in some fast-food restaurants in Batna city (Algeria) is evaluated. To assess the oils quality, the following physicochemical parameters are analyzed: refractive index, density, viscosity, smoke point, the peroxide value, the acid value and the level of polar compounds which were applied in order to control sample prepared in the laboratory and those collected from ten fast food restaurants. The results showed that two out of ten samples of different fast-food restaurants are unfit for frying and must be renewed and this taking into account the results of most parameters that exceed the recommended specifications, while taking into account the level of polar compounds, four out of ten of the samples are non-compliant with the standards recommended for oils. Thus, they have either been used more than nine times or in frying adverse conditions. For that, the frying remains an operation simple to be made, but complex to be mastered, seen the reactions interfering between the oil and the environmental factors as well as between the oil and the food. So it’s essential to focus rather on the frying conditions.
IN VITRO STUDY OF BIOCONTROL POTENTIAL OF RHIZOSPHERIC MICROORGANISMS AGAINST FUSARIUM OXYSPORUM F.SP. ALBEDINIS
(Pakistan Journal of Phytopathology, 2022-06-25) Anis BERTELLA
The present work investigated the ability of antagonistic microorganisms to inhibit the growth of pathogenic strains responsible for the vascular fusarium wilt of date palm or Bayoud's disease. The objective was to evaluate the antagonistic effect of native strains of Trichoderma longibarchiatum, Penicillium sp., Fusarium sp. and one isolate of Bacillus sp. against four isolates of Fusarium oxysporum f.sp. albedinis (Foa) under in vitro conditions. The isolates Bacillus sp. and Fusarium sp. were isolated from soil samples of the rhizosphere of date palm of Tamanrasset region in Algeria, these strains were morphologically identified, and a dual culture confrontation was carried out, placing the Foa in the center of the Petri dish and Bacillus sp. in the two cardinal points. For T. longibarchiatum, Penicillium sp. and Fusarium sp. they were confronted against Foa with a dual confrontation, the inhibition rates were determined, and a percentage of inhibition obtained varied from 64.29 to 65.75%; 57.14 to 64.29% and 36.36 to 52.05% for T. longibarchiatum, Penicillium sp and Fusarium sp. respectively. While Bacillus sp. exhibits the lowest percentage of inhibition in this study, and they were less than 16.67%. Comparison of mean value for each pair between T. longibrachiatum, Penicillium sp., Fusarium sp. on one hand and Bacillus sp. on the other hand concluded that there is a highly significant difference between these three fungal isolates and Bacillus sp. concerning inhibition rates with P-value (P <0.0001). Contrary, no significant difference was observed between T. longibrachiatum and Penicillium sp. with P = 0.1895 and the two antagonists show the same letter A in the letter connection ratio, which means no difference in the level of inhibition.
Influence of medicinal and aromatic plants into risk assessment of a new bioactive packaging based on polylactic acid (PLA)
(elsevier, 2019-07-03) Anis BERTELLA
A new biodegradable antioxidant active packaging for food applications based on antioxidants from medicinal and aromatic plants incorporated into a polylactic acid matrix was designed and developed. Melt blending processing technique was applied to prepare polylactic acid films loaded by sage and lemon balm leaves. Antioxidant properties of developed active films were investigated using the following methods: 2,2′-azino-bis(3- ethylbenzothiazoline-6-sulphonic acid), 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl and a home-made generator of hydroxyl radicals. In addition, reducing power and total phenolic content of polylactic acid films were checked. The results of antioxidant capacity showed that percentage of hydroxylation for active film with lemon balm and sage was 55.5% ± 0.1% and 67.4% ± 0.3%, respectively. The reducing power of active films increased 8 times in comparison to the blank samples. Moreover, extensive investigation of influence of sage and lemon balm leaves on material safety and type of migrants was performed using migration assays. The composition of both non-volatile and volatile compounds of different active packaging films was compared with neat polylactic acid film. Three different food simulants such as 95% (v/v) ethanol, 10% (v/v) ethanol and 3% (w/v) acetic acid were checked. It was shown that the addition of sage and lemon balm leaves into a polylactic acid structure decreased the migration of both linear and cyclic polylactic acid oligomers, currently not legislated by European Union. Besides, total absence or decrease of migration of volatile compounds were observed when using the active films. Both thermal and mechanical properties of films were also evaluated.
Morphological study and Caracterisation of Fusarium oxysporum f.sp.albedinis. by Isozymes systems
(International Journal of Scientific & Engineering Research, 2017-01-01) Anis BERTELLA
Abstract— The characteristics of Fusarium oxysporium f.sp. albedinis (F.o.a) isolates were investigated using electrophoretic studies of isozymes systems (esterase and phosphatase). The morphological characteristics of the isolates were very variable to each other. The isoenzymes profiles revealed polymorphic bands or the data were subjected to analysis with the JMP method. The isolates were grouped into 2 main groups A and B, those last were divided into sub-groups. Nineteen (19) isolates creates the group A and four isolates (E1, E2, E3 and M15A) formed the group B. analysis of isozyme banding patterns were found to be a reliable marker technology, efficient and effective tools to find the genetic variability among isolates isolated in different geographical areas.
Pathogenicity and biological control of Bayoud disease by Trichoderma longibrachiatum and Artemisia herba-alba essential oil
(Journal of Applied Pharmaceutical Science, 2018-04-29) Anis BERTELLA
All the Fusarium oxysporum f. sp. albedinis (Foa) isolated from rachis were pathogenic to the date palm seedlings cultivar Deglet Nour, while Fusarium sp. isolated from soil (E1, E2, and E3) did not show any aggressiveness against these seedlings. In vitro antagonistic effect of Trichoderma longibrachiatum against three isolates of Foa tested by direct confrontation or remote confrontation on Potato Sucrose Agar (PSA) medium, revealed that the latest has inhibited mycelial growth of the pathogen by more than 60%, compared to the control and this after an incubation period of six days at 27 ± 2°C. Mycelial growth of T. longibrachiatum occupied the whole Petri dish after three days of incubation while the three isolates of Foa (T15D, M15A1, and O15T) occupied only 17%, 11%, and 20%, of the surface respectively. Biological control with essential oil of Artemisia herba-alba yielded important results with a minimum inhibitory concentration (MIC) ranging from 2.5 to 5 μl/ml and a minimum fungicidal concentration (MFC) of 80 μl/ml.
PRELIMINARY STUDY OF ANTIMICROBIAL ACTIVITY AND PHENOTYPIC CHARACTER FROM ACTINOMYCETES AND PENICILLIUM SP
(International Journal of Pharmaceutical Sciences and Research, 2023-06-01) Anis BERTELLA
ABSTRACT: Indeed, Actinomycetes and Penicillium are microorganisms that live in different ecosystems. Besides, they play important roles in various fields in respect such as agriculture, medicine and the environment. During this study, we were interested in the isolation of two strains namely: Actinomycetes and Penicillium sp. from coconuts and truffle respectively. In addition, the agar cylinder technique demonstrated the antibacterial activity of Penicillium sp. against four pathogenic bacterial strains (S. aureus, E. coli, P. aeruginosa and K. pneumoniae). In virtue of which, the results showed a variability of the diameters of the inhibition zones, thus the most important results were recorded against K. pneumoniae and S. aureus, which produced inhibition zones of 17.50±0.71 mm and 17.00±0.00 mm respectively. In order to struggle against pathogenic fungal strains, the ability of Actinomycete strain to suppress the mycelial growth of 03 pathogenic strains of Fusarium oxysporum f.sp. albedinis (EF1, EF2 and EF3) was examined. Hence, the result of the direct confrontation tests on the PDA medium between the Actinomycete strain and these pathogenic strains revealed a significant inhibition of the mycelial growth of EF3 (62,5%) and EF2 (52,70%). On the other hand, the lowest inhibition rate was recorded in EF1 (34.55%).
TOTAL PROTEIN POLYMORPHISM OF SOME ISOLATES OF FUSARIUM OXYSPORUM F. SP. ALBEDINIS USING SDS-PAGE
(Pakistan Journal of Phytopathology, 2021-06-19) Anis BERTELLA
Fusarium wilt caused by Fusarium oxysporum f.sp. albedinis (Foa), is one of the main diseases affecting date palm (Phoenix dactylifera L.), and poses a constant threat to palm oases, especially in North African countries. In this study, we performed electrophoresis of total proteins extracted from the mycelia of 20 Fusarium oxysporum f.sp. albedinis and 03 isolates of Fusarium sp. Protein was extracted and separated by Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis followed by staining with silver nitrate. We noted variations in protein profile between the 23 Fusarium strains isolated from three regions Ghardaïa, Bechar and Adrar. These results demonstrate the presence of important phenotypic polymorphism according to the ascending hierarchical classification of total proteins after the analysis of the phonograms constructed by the JMP software (JMP SAS Pro (JMP®, Version <12>. SAS Institute Inc., 2015).

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